雌孕激素通过调节一氧化氮影响束缚应激小鼠结肠动力

Estradiol and Progesterone Regulate the Activities of Colonic Smooth Muscle in Water-immersion and Restraint Stress Mice by Modulating NO

目的:应激对胃肠动力有重要的影响,可引起肠易激综合征患者症状的发作或加重,本研究采用束缚-浸水应激诱导肠易激综合征模型小鼠,分别予以雌激素、孕激素和雌激素+孕激素处理,观察结肠动力学及NO含量指标,以探讨雌、孕激素能否改善应激诱发的结肠功能异常并探讨其调节机制,进从为临床治疗肠易激综合征提供理论依据。方法:建立束缚浸水应激诱发的肠易激综合征小鼠模型,制备离体结肠平滑肌肌条,应用张力换能器测定其肌张力,并检测其排便粪点数以观察结肠平滑肌动力,采用硝酸还原酶法测量结肠一氧化氮(NO)的含量。结果:束缚浸水应激刺激诱发结肠肌张力增加和NO含量减少,与模型组比较,雌激素可逆转该作用,而孕激素、雌激素+孕激素则使该作用增强。结论:雌孕激素可以通过调节NO含量变化影响束缚-浸水应激诱导的肠易激综合征结肠收缩活动。束缚-浸水应激导致肠易激综合征模型小鼠结肠动力学改变,表现为增强远端结肠平滑肌收缩活动,同时模型小鼠结肠NO含量降低;雌激素可以逆转结肠平滑肌收缩活动增强和结肠NO含量降低;孕激素、雌激素+孕激素使结肠平滑肌收缩活动增强和结肠NO含量降低作用更加明显。

Objective： Stress affects gastrointestinal motility ang causes more severe symptoms of irritable bowel syndrome.This study adopts water-immersion and restraint stress mice model of irritable bowel syndrome and observe effect of estradiol and progesterone on the colon mobility and changes of nitric oxide in the colon.Then we investigate the regulatory effect of estradiol and progesterone on the colon mobility and its mechanism and provide the theory basis for clinical treatment IBS.Methods： The irritable bowel syndrome mice model was induced by Water-immersion and restraint stress.The muscular tension was measured by observing dung points and the muscular tension of isolated muscle strips from colon in mice was measured by transducer.Nitrate/nitrite colorimetric assay was used to detect the NO content in colon.Results： Water-immersion and restraint stress increased the colon mobility and reduced colon NO level.Compared with the model group,estradiol partly reversed the above effects.Progesterone and estradiol＋progesterone intensify these effects.Conclusion： Estradiol and progesterone regulate the activities of colonic smooth muscle in Water-immersion and restraint stress mice by modulating NO.The intensity of colon mobility evaluated and the content of nitric oxide in the colon reduced in the water-immersion and restraint stress mice model of irritable bowel syndrome.Estradio reverses the above effects and progesterone strengthen the above effects.