抗凋亡蛋白Survivin和Ki-67在肝癌组织中的表达及相关性研究

The Expression and Clinical Significance of Survivin and Ki-67 in Primary Hepatocellular Carcinoma

目的:观察抗凋亡蛋白Survivin和Ki-67在原发性肝癌(PHC)中的表达并探讨其临床意义。方法:采用免疫组织化学S-P法检测34例原发性肝癌组织、14例癌旁正常肝组织中survivin和Ki-67的表达情况。结果:原发性肝癌组织中survivin的阳性表达定位于细胞浆和细胞核,阳性表达率明显高于癌旁正常肝组织(P<0.05);Ki-67的阳性表达主要定位于细胞核,其阳性表达率亦明显高于癌旁正常肝组织(P<0.05)。二者的阳性表达与原发性肝癌患者的性别、年龄、肿瘤大小无相关性(P>0.05),与淋巴结转移、组织分化程度及癌栓是否形成有关(P<0.05);此外,二者在原发性肝癌组织中的表达呈显著正相关(P<0.05)。结论:Survivin和Ki-67在原发性肝癌组织中表达上调,均与原发性肝癌的组织分化程度及癌栓是否形成密切相关;检测survivin和Ki-67的表达有助于原发性肝癌的预防、治疗和预后评估。

Objective： To observe the expression of survivin and Ki-67 in primary hepatocellular carcinoma and analyze their clinical significances.Methods： The expression of survivin and Ki-67 in 34 cases of hepatocellular cancer,14 cases of adjacent normal liver tissue were detected by immunohistochemistry（S-P）.Results： The positive expression of survivin located in cytoplasm and nucleus,which was significantly higher in primary hepatocellular carcinoma than in normal tissue（P0.05）;the positive expression of Ki-67 mainly located in nucleus,which was also significantly higher in primary hepatocellular carcinoma than in normal tissue（P0.05）.Both expression of survivin and Ki-67 were unrelated to the sex,age and size of tumor of patients（P0.05）,but they were related with lymph node metastasis,the degree of histological differentiation and formation of tumor thrombus（P0.05）.Positive relationship was found between the expression of survivin and Ki-67 in primary hepatocellular carcinoma（P0.05）.Conclusion： Both the expression of survivin and Ki-67 upregulated in primary hepatocellular cancinoma,which were closely related to the degree of histological differentiation and formation of tumor thrombus.Detection of the expression of survivin and Ki-67 may contribute to the prevents,treatment and prognosis evaluation of primary hepatocellular cancinoma.