细胞周期调控蛋白p21^(WAF1)在腹膜间皮细胞肥大中的作用

Effect of cell cycle regulator p21^(WAF1) on hypertrophy of peritoneal mesothelial cells

目的 :观察p2 1WAF1在高糖作用下腹膜间皮细胞肥大中的作用。方法 :用RT -PCR和WesternBlot法测定在高浓度葡萄糖 (含糖为 1 38%、3 86 % )作用 2 4h后体外培养的大鼠腹膜间皮细胞p2 1WAF1水平 ;用流式细胞仪分析细胞周期分布。结果 :高糖作用 2 4h后大鼠腹膜间皮细胞大多出现细胞肥大和停滞于细胞周期的G1期 ,以含糖 3 86 %组明显。p2 1WAF1mRNA和蛋白随着糖浓度增高表达增高 (含糖组间P <0 0 5 )。在和含糖组相同渗透压的甘露醇组和无血清常规培养基几乎无p2 1WAF1mRNA和蛋白的表达。结论 :p2

AIM: To investigate the effect of cell cycle regulator p231 WAF1 on hypertrophy of peritoneal mesothelial cells affected by high concentrated glucose. METHODS: RT-PCR and Western Blot method were used to detect p21 WAF1 expression of rat peritoneal mesothelial cells in high glucose concentration medium (containing 1 86%, 3.86% glucose) after 24 hours. Flow cytometer technique was used to analyze the cell cycle distribution. RESULTS: In high glucose medium, most of the cells became hypertrophy, and were arrested in G1 phase of the cell cycle, which was obvious in 3.86% glucose group. Glucose increased p21 WAF1 mRNA and protein expression in a dose-dependent manner, and the levels of p21 WAF1 mRNA and protein in 3.86% glucose group were higher than those in 1.38% glucose group ( P<0.05 ). p21 WAF1 mRNA and protein expression were absent in the serum-free normal medium and D-mannitol groups which had the same osmolarity as the glucose groups. CONCLUSION: p21 WAF1 may be pivotal in the hypertrophy and arrest in the G1 phase of mesothelial cells induced by high concentrated glucose.