胡萝卜愈伤组织上长期培养和红掌上复壮繁殖对相似穿孔线虫耐低温能力的影响

The Tolerance Ability to Low Temperature of Radopholus similis Reduced Cultured Longterm on Carrot Callus and Recovered Rejuvenated on Anthurium andraeanum

利用从观赏植物上截获的相似穿孔线虫Radopholus similis 6个种群,研究在胡萝卜愈伤组织上长期培养繁殖和在红掌Anthurium andraeanum上接种繁殖复壮后,相似穿孔线虫耐低温能力的变化.将经2种方式培养繁殖的相似穿孔线虫接种在胡萝卜愈伤组织上,测定其耐低温能力.结果表明,在胡萝卜愈伤组织上长期(6代)连续培养后,相似穿孔线虫的耐低温能力降低,在低温下的生存能力和繁殖能力明显降低;通过在自然寄主植物红掌根系上接种繁殖后,相似穿孔线虫在低温下的生存和繁殖能力又可得到不同程度的恢复,即耐低温能力得到复壮.胡萝卜愈伤组织上长期培养繁殖对相似穿孔线虫不同群体耐低温能力的影响不同,相似穿孔线虫不同种群在红掌上繁殖后耐低温能力的恢复程度也有所不同.

The enduring fitness to low temperature of six isolates of Radopholus similis from ornamental plants was studied through longterm culture propagation on carrot callus and rejuvenation culturing on Anthurium andraeanum.The tolerance to low temperature of R.similis was determined by culturing on carrot callus.The results showed that the tolerance to low temperature of R.similis reduced after successive culturing on carrot callus for six generations,and the survival and fecundity decreased significantly.After rejuvenation on A.andraeanum,the survival and fecundity of R.similis could recover in different degrees,namely the resistant capacity to low temperature was enhanced.The tolerance to low temperature of R.similis was different in longterm cultured carrot callus according to the varying nematode groups.Similarly,the degree of recovery of the resistance to low temperature was also different after being cultured on A.andraeanum.