建立大鼠深低温肺缺血再灌注在体模型的实验研究

Experimental Study of Establishment of in vivo Rat Deep Hypothermia Lung Ischemia-reperfusion Model

目的建立一种新的模拟深低温停循环条件下肺缺血再灌注大鼠模型。方法 Wister大鼠30只,分为实验组、对照组和正常组。麻醉后气管切开插管接小动物呼吸机,股动脉置管测动脉压力,监测心电、肛温、血氧饱和度和血气分析,用体外降温至肛温24℃。实验组阻断左下肺门30min后开放,并逐渐将体温恢复正常;对照组单纯降温复温。复温后处死动物,取肺组织标本计算W/D值及病检。结果实验大鼠无死亡。实验组W/D值较正常组和对照组明显增高(P<0.05)病检显示实验组明显肺泡间质水肿,大量炎性细胞、红细胞渗出;而对照组病变明显减轻。结论 本实验方法制作的大鼠模型可以用于模拟人深低温停循环肺缺血再灌注的研究。

Objective To establish the new rat model of deep hypothermic circulatory arrest （DHCA） with lung ischemia - reperfu- sion. Methods Thirty Wister rats were divided into experimental group, control group and normal group. Anesthetized rats received tra- eheostomy ventilation. Femoral artery catheter was placed to measure artery pressure,ECG was monitored. Oxygen saturation and blood gas analysis was done, and rectal temperature with external cooling to 24~C was recorded. Left lower pulmonary artery occlusion in experimen- tal group （the control group did not block） will gradually return to normal body temperature. After the body temperature restored, rats were put to death and lung tissue samples were taken to calculate W/D and pathological examination Results No rat failed. Compared to normal group and control group, W/D value in the experimental group increased significantly （P 〈 0.05 ）. It＇s pathologic examination showed marked alveolar interstitial edema, majority inflammatory cells＇ and red blood cell leakage. Conclusion The method can be used to simulate people to deep hypothermic circulatory arrest in lung ischemia- reperfusion.