计算机图像分析行肾结晶评分的可行性研究

Feasibility Calculating of the Renal Crystallization Score with Computer Image Analysis

目的探索计算机图像软件Image-Pro.Plus(IPP)在肾结晶评分中的可行性。方法对Olympus IX-51显微镜获取的4组40张HE染色的肾切片图像用IPP进行结晶物理参数测量,筛选出最佳参数,并与结晶的人工镜下直接评分进行稳定性和可靠性的比照。结果结晶面积是IPP测量的最佳参数,可实现结晶评分的无级量化,稳定性和可靠性均优于人工分级评分法。结论 IPP结晶评分具有良好的精确性、可靠性及稳定性,尤其适于批量处理。

Objective To stuly the feasibility of calculating the renal crystallization score with computer image analysis. Methods Forty HE staining slice were divided into four groups. Olympus - IXS1 microscope was used to obtain these slices picture. These images were test with software IPP and the crystallization information of physical measurement parameter were obtained. The best score parameter was filter out and stability and reliability compared were with artificial direct endoscopic score of crystallization. Results Area of crystalli- zation was the best parameter in the measurement of software IPP. IPP crystallization score could obtain the parameter information of crys- tallization full quantitatively and its stability and reliability were much better than artificial grading score. Conclusion IPP crystallization score has good accuracy, reliability and stability, which is especially suitable for the crystallization of the score batch processing.