七氟醚对单肺通气致肺损伤兔肺组织胞质型磷脂酶A_2和clara细胞分泌蛋白表达的影响(英文)

Effects of sevoflurane on pulmonary cytosolic phospholipase A_2 and clara cell secretory protein expressions in rabbits withone-lung ventilation-induced lung injury

目的探讨七氟醚对单肺通气(OLV)兔肺组织胞质型磷脂酶A2(C-PLA2)及clara细胞分泌蛋白(CCSP)表达的影响。方法36只健康日本大耳白兔随机分为假手术组(S组)、OLV组(O组)和OLV+七氟醚组(OS组)。OS组据所给七氟醚浓度(体积分数)不同分为1%、2%、3%和4%亚组。Western blotting和定量PCR分别用于检测肺组织中CCSP和C-PLA2蛋白及mRNA的表达水平。ELISA检测肺组织花生四烯酸(AA)含量。通过观察肺湿/干(W/D)和肺组织的形态学改变评价肺损伤的严重程度。结果 OLV后兔肺组织CCSP蛋白和mRNA表达水平明显降低(P<0.05),而C-PLA2蛋白和mRNA表达水平、肺组织AA含量和肺损伤程度均明显增加(P<0.05);OLV+七氟醚组中肺组织CCSP蛋白和mRNA表达增加(P<0.05),而C-PLA2蛋白和mRNA表达水平和肺组织AA含量降低(P<0.05),肺损伤减轻(P<0.05);OLV+不同浓度七氟醚组中肺组织CCSP蛋白和mRNA表达水平无显著性差异,但肺组织C-PLA2蛋白和mRNA表达水平、肺组织AA含量和肺损伤程度随浓度的增加而逐渐降低(P<0.05)。结论 OLV可使兔肺组织中CCSP蛋白和mRNA表达水平降低,肺组织C-PLA2蛋白和mRNA表达水平和AA含量增加,引起急性肺损伤。七氟醚可通过逆转OLV的上述效应而发挥抗急性肺损伤作用。

Objective To investigate the effects of sevoflurane on cytosolic phospholipase A2（C-PLA2） and clara cell secretoryprotein（CCSP） in lung tissues of rabbits with one-lung ventilation（OLV）-induced lung injuries.Methods Thirty-six healthyJapanese white rabbits were randomized into sham-operated group,OLV group,and OLV plus sevoflurane group subdividedinto 4 subgroups with sevoflurane concentrations of 1%,2%,3% and 4%.CCSP and C-PLA2 mRNA and protein expressions inrabbit lung tissues were detected by Western blotting and real-time PCR,and the content of arachidonic acid（AA） wasmeasured using ELISA.The severities of the lung injury were evaluated according to lung wet/dry weight（W/D） ratio andhistological scores.Results In the OLV group and OLV＋ sevoflurane groups,pulmonary CCSP expressions were significantlylower,while C-PLA2 expression,lung W/D ratios and lung histological scores were significantly higher than those in thesham-operated group（P0.05）.Compared with OLV group,the OLV ＋ sevoflurane groups showed significantly increasedexpressions of CCSP and reduced C-PLA2 expression,lung W/D ratios and histological scores（P0.05）.In the 4 OLV ＋sevoflurane groups,CCSP expressions underwent no significant changes as sevoflurane concentration increased,but C-PLA2expressions,lung W/D ratios and histological scores all decreased gradually as the concentrations of sevoflurane increased（P0.05）.Conclusion OLV can result in down-regulated CCSP expressions andup-regulated C-PLA2 expressions in rabbit lungtissues.Sevoflurane can protect against OLV-induced acute lung injury possibly by inhibiting C-PLA2 expression viaup-regulation of CCSP expressions or through other mechanisms resulting in down-regulated expression of C-PLA2.