COX-2、PKC-α和miR-101在胃癌中的表达及相关性

Expressions of COX-2,PKC-α and miR-101 in gastric cancer and their correlations

目的探讨miR-101、蛋白激酶C-α(PKC-α)和环氧合酶2(COX-2)在胃癌中的表达及相关性。方法(1)荧光定量RT-PCR法检测57例胃癌组织标本对应的正常胃粘膜标本中miR-101的表达水平;(2)Western blotting检测57例胃癌组织标本对应的正常胃粘膜标本中PKC-α蛋白和COX-2蛋白的表达水平。结果(1)胃癌组织中miR-101表达明显低于正常胃粘膜(P<0.05);胃癌组织中COX-2及PKC-α蛋白表达高于正常胃粘膜(P<0.05);(2)胃癌中COX-2蛋白的表达与其分化程度、浸润深度、淋巴结转移、肿瘤病理分期有关(P<0.05);胃癌中PKC-α蛋白的表达与肿瘤分期和淋巴转移有关(P<0.05);(3)胃癌组织中COX-2蛋白相对表达水平与胃癌组织中PKC-α蛋白表达呈正相关,与胃癌组织中miR-101表达呈负相关,两者相关具有统计学意义(r=0.531,P<0.05;r=-0.627,P<0.05)。结论 miR-101、PKC-α和COX-2可能参与了胃癌的发生发展;miR-101和PKC-α可能是抑制COX-2在胃癌中表达的新靶点。

Objective To investigate the expressions of miR-101,protein kinase C-α（PKC-α）,and cycloxygenase-2（COX-2） in gastric cancer（GC） tissue and their correlations.Methods RT-qPCR was used to examine miR-101 expression and Western blotting employed to detect PKC-α and COX-2 expressions in 57 cases of gastric cancer tissues and paired normal gastric mucosal tissues.Results The gastric cancer tissues showed a significantly lower miR-101 expression（Z=6.102,P0.05） but significantly higher expressions of COX-2（Z=14.436,P0.05） and PKC-α（Z=6.955,P0.05） than the normal gastric tissues.The expression of COX-2 protein was significantly correlated with the degree of differentiation,invasion depth,lymph node metastasis and TNM stage（P0.05）;PKC-α protein expression was associated with lymph node metastasis and TNM stage（P 0.05）.PKC-α expression was positively correlated（r=0.531,P0.05） and miR-101 expression negatively correlated（r=-0.627,P 0.05） with COX-2 expression in gastric cancer tissues.Conclusion miR-101,PKC-α and COX-2 all play a role in the tumorigenesis and progression of gastric cancer.miR-101 and PKC-α might be new potential therapeutic targets for inhibiting COX-2 in gastric cancer.