急性缺血性脑卒中介入取栓器血栓模型的机械特性比较

Comparison of mechanical characteristics of blood clot models used for the evaluation of interventional thrombectomy devices for acute stroke

目的比较用于评估治疗急性缺血性脑卒中的介入取栓器械的血栓模型。为介入机械取栓器械的研制、评估提供更为科学的血栓模型。方法复制Kan及Gralla的可透X线血栓模型,自制聚氯乙烯管内自发凝固成型可透X-线红色血栓模型(spontaneous non-radiopaque tube red clots,SNTR)及聚氯乙烯管内自发凝成型可透X-线的白色血栓模型(spontaneous non-radiopaque tube white clots,SNTW)。用弹性实验,拉伸实验评价比较血栓模型的机械力学性能。用HE染色评价血栓模型的组织结构。结果制备出SNSW(Kan模型)、TNTR(Gralla模型)、SNTR及SNTW 4种机械特性完全不同的可透X-线血栓模型,在24 h及1和2周的弹性实验(度)结果分别为SNSW:5.00±4.47,4.16±3.76,5.83±6.64;TNTR:19.33±6.05,16.66±4.08,19.16±9.70;SNTR:10.00±7.07,7.50±6.89,13.33±4.08;SNTW:87.5±2.73,81.66±6.83,88.33±4.08。拉伸实验(cm)的结果分别为SNSW:4.43±0.38,4.67±0.29,4.60±0.31;TNTR:3.79±0.13,3.91±0.11,3.91±0.16;SNTR:3.25±0.15,3.46±0.10,3.33±0.27;SNTW:4.22±0.18,4.40±0.32,4.11±0.15。结论 SNSW,TNTR,SNTR及SNTW为4种机械性能不同的血栓模型,根据研究目的及器械的取栓原理从4种血栓模型中选用或联合其中几种评估介入取栓器械的效能更为合理。

Objective To compare the mechanical characteristics of blood clot models used for the evaluation of interventional thrombectomy devices for acute stroke, and to provide more scientific blood clot models for the manufacture of interventional thrombectomy devices. Methods The Kan＇s and Gralla＇s radioparent tube clots model was duplicated. The spontaneous radioparent tube red clots （SNTR） and spontaneous radioparent tube of white clots （SNTW） were prepared. By bending testing and elongation testing the mechanical properties of clot models were evaluated. By using HE staining the organizational structure of the clot models was assessed. Software SAS9.3 was used to analyze the data. Results SNSW （Kan model）, TNTR （Gralla model）, SNTR and SNTW were successfully prepared. The bending testing of the four radioparent clot models that carried quite different mechanical properties was separately performed at 24 hours, one and two weeks later. The results showed that the elasticity of SNSW was （5.00 ± 4.47）, （4.16 ± 3.76） and （5.83 ± 6.64） degree respectively; the elasticity of TNTR was （19.33 ± 6.05）, （16.66 ± 4.08） and （19.16 ± 9.70） degree respectively; the elasticity of SNTR was （10.00 ± 7.07）, （7.50 ± 6.89） and （13.33 ± 4.08） degree respectively; and the elasticity of SNTW was （87.5 ± 2.73）, （81.66 ± 6.83） and（88.33 ± 4.08） degree respectively. The elongation testing of the four radioparent clot models that carried quite different mechanical properties was separately performed at 24 hours,one and two weeks later. The results showed that the stretch limit of SNSW was （4.43 ± 0.38）, （4.67 ± 0.29） and （4.60 ± 0.31 ） cm respectively; the stretch limit of TNTR was （3.79 ± 0.13）, （3.91 ± 0.11 ） and （3.91 ± 0.16） cm respectively; the stretch limit of SNTR was （3.25 ± 0.15）, （3.46 ± 0.10） and （3.33 ± 0.27） cm respectively; the stretch limit of SNTW was （4.22 ± 0.18）, （4.40 ± 0.32） and （4.11 ± 0.15） cmrespectively. Conclusion SNSW, TNTR SNTR and SNTW are four clot models with different mechanical properties. It is reasonable that the use of one proper clot model or some of these clot models should be based on the purpose of the study.