期刊文献+

c-Jun/AP-1在因子Ⅶa促进SW620细胞增殖和迁移中的激活及其调控作用 被引量:3

Activation and regulation of c-Jun/AP-1 in factor Ⅶa-induced proliferation and migration of SW620 cells
原文传递
导出
摘要 目的探讨c-Jun/激活蛋白1(AP-1)在活化的凝血因子Ⅶa促进SW620细胞增殖、迁移过程中的作用及其调控机制。方法 Western blot检测人结肠癌SW620细胞中Ⅶa、组织因子(TF)、蛋白酶激活受体2(PAR2)、细胞外调节蛋白激酶1/2(ERK1/2)抑制剂U0126、p38抑制剂SB203580处理后c-Jun、磷酸化c-Jun(p-c-Jun)的蛋白水平变化;MTT和Transwell法分别检测AP-1抑制剂姜黄素对细胞增殖和细胞迁移能力的影响。结果单克隆抗TF和PAR2抗体、U0126均能抑制Ⅶa促进SW620细胞中c-Jun/AP-1活化的过程(P<0.05)。姜黄素降低Ⅶa诱导的SW620细胞增殖和细胞迁移能力(P<0.05)。结论 TF/Ⅶa复合物通过激活PAR2促进SW620细胞增殖和迁移,c-Jun/AP-1在此过程中被激活并发挥重要作用,ERK1/2为c-Jun/AP-1上游分子具有调控效应。 Objective To irvesfigate the effect and underlying mechanism of c-Jun/activator protein-l(AP-1) in factor Ⅶa-indUced proliferation and migration of SW260 cells. Methods The protein expressions of c-Jun and p-c-Jun treated with Ⅶa, tissue factor (TF), protease-activated receptor 2(PAR2), ERK1/2 inhibkor U0126 and p38 inhibitor SB203580 in colon cancer cell line SW620 were detected by Western blot. The proliferation and migration of SW620 cells were measured by MTT and Transwell assays, respectively. Results Factor Ⅶa promoted the protein expressions of c-Jun and p-c-Jun in a time-dependent manner, which was inhibited by anti-TF, anti-PAR2 antibodies and U0126 (P〈0. 05). Cureumin attenuated Ⅶa-induced cell proliferation and migration in vitro (P〈0. 05). Conclusion TF/Ⅶa complex enhances the proliferation and migration of SW620 cells via PAR2 activity, during which c-Jun/AP-1 is activated and regulated by the upstream molecule of ERK1/2.
出处 《江苏医药》 CAS 北大核心 2013年第7期764-767,共4页 Jiangsu Medical Journal
基金 江苏省自然科学基金(BK2010336) 江苏大学学生科研立项(Y11A183)
关键词 结肠癌 凝血因子Ⅶa 蛋白酶激活受体2 激活蛋白1 Colon cancer Blood coagulation factor Ⅶa Protease-activated receptor 2 Activator protein-1
  • 相关文献

参考文献2

二级参考文献47

  • 1Schrer K, Bretschneider E, Fischer K, Fischer JW, Pape R, Rauch BH, et al. Thrombin receptors in vascular smooth muscle cells -- function and regulation by vasodilatory prostaglandins. Thromb Haemost 2010; 103: 884-90.
  • 2Steinhoff M, Buddenkotte J, Shpacovitch V, Rattenholl A, Moormann C, Vergnolle N, et al. Proteinase-activated receptors: transducers of proteinase-mediated signaling in inflammation and immune response. Endocr Rev 2005; 26: 1-43.
  • 3Martorell L, Martinez-Gonzalez J, Rodriguez C, Gentile M, Calvayrac O, Badimon L. Thrombin and protease-activated receptors (PARs) in atherothrombOsis. Thromb Haemost 2008; 99: 305-15.
  • 4Dabbagh K, Laurent G J, McAnulty R J, Chambers RC. Thrombin stimulates smooth muscle cell procollagen synthesis and mRNA levels via a PAR-1 mediated mechanism. Thromb Haemost 1998; 79: 405-9.
  • 5Perbal B. CCN proteins: multifunctional signalling regulators. Lancet 2004; 363: 62-4.
  • 6Bradham DM, Igarashi A, Potter RL, Grotendorst GR. Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-IO. J Cell Biol 1991; 114: 1285-94.
  • 7Blom IE, Goldschmeding R, Leask A. Gene regulation of connective tissue growth factor: new targets for antifibrotic therapy? Matrix Biol 2002; 21: 473-82.
  • 8Cicha I, Yilmaz A, Klein M, Raithel D, Brigstock DR, Daniel WG, et al. Connective tissue growth factor is overexpressed in complicated atherosclerotic plaques and induces mononuclear cell chemotaxis in vitro. Arterioscler Thromb Vasc Biol 2005; 25: 1008-13.
  • 9Ruperez M, Lorenzo O, Blanco-Colio LM, Esteban V, Egido J, Ruiz- Ortega M. Connective tissue growth factor is a mediator of angio- tensin II-induced fibrosis. Circulation 2003; 108: 1499-505.
  • 10Ruiz-Ortega M, Rodr~guez-Vita J, Sanchez-Lopez E, Carvajal G, Egido J. TGF-[3 signaling in vascular fibrosis. Cardiovasc Res 2007; 74: 196-206.

共引文献43

同被引文献32

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部