凋亡相关基因BAG-1在脑膜瘤中的表达及意义

The expression and significance of apoptosis related gene BAG-1 in meningioma

目的:通过检测人类脑膜瘤中凋亡相关基因BAG-1的表达,以进一步了解脑膜瘤的生物学行为。方法:对158例脑膜瘤病理标本采用链霉菌抗生物素蛋白-过氧化物酶连结法(SP),以单克隆抗体直接检测BAG-1的表达情况。按染色强度和阳性范围评估免疫组化检测结果,分析BAG-1表达程度与脑膜瘤病理分级、术后复发的相关关系。结果:同一WHO2007病理分级的各亚型以及Ⅱ级、Ⅲ级之间的各亚型,在BAG-1表达程度上,不具有差异性(P>0.05);病理分级Ⅰ级中的亚型与Ⅱ级、Ⅲ级中的亚型,在BAG-1表达程度上具有差异性,其差异具有统计学意义(P<0.05或P<0.01);随着WHO病理分级增高,脑膜瘤BAG-1表达的免疫组化评分逐渐下降,其结果具有统计学意义(χ2=141.49,P<0.01);随着脑膜瘤BAG-1表达的免疫组化评分下降,脑膜瘤术后较易复发,其结果具有统计学意义(χ2=55.13,P<0.01)。结论:脑膜瘤BAG-1的表达与其WHO病理分级相关,BAG-1表达越低,其术后越易复发。

Objective： To analyze the expressions of BAG-1 in meningioma for further understanding of biological behaviors of meningiomas. Methods： The specimens included in this study were collected from 158 meningioma eases. Streptavidin-pemsidase were used in immunohistochemical staining. the results of immunohistochemical score were depending on the positive ratio and intensity of the immunoreacfivity. The expressions of BAG-1 in meningioma were analyzed in relationship with histopathologic grading,postoperative recurrence. Results： The difference in the expression degree of BAG- 1 between each subtype in the same histopathologic grade and various subtypes between the grade of Ⅱ to Ⅲwere not statiscally significant（ P 〉 0.05）. The expression degree of BAG-I between each subtype in the pathological grade 1- to the each subtype pathological grade [1 or m was different, the difference had statistical significance（ P 〈 0.05 or P 〈 0.01 ）. The immunohisto- chemical score of the expression of BAG-1 was decreased gradually with the pathologic grading of WHO increased, and the result was statistically significant （X2= 141.49, P 〈 0.01 ）. As the immunohistochemieal score of the expression of BAG-1 decreased the postoperative meningioma was easy to recure, the result was statistically significant （X2 = 55.13, P 〈 0.01）. Conclusion： The expression degree of BAG- 1 is in dose correlations with the WHO pathologic grading of meningioma. The lower the expressions of BAG- 1, the more recurrent with pestoperation of meningiomas will be.