急性肾衰竭家兔红细胞流变性变化

Changes of erythrocyte rheology in rabbits with acute renal failure

目的:观察急性肾衰竭(ARF)家兔红细胞流变性变化。方法:38只健康家兔分为对照组(n=8)与模型组(通过肌肉注射1%HgCl2复制ARF模型,分为12 h、24 h、48 h三个亚组,n=10)。在相应时间点,乌拉坦麻醉后,经颈动脉插管留取动脉血标本,检测肾功能与红细胞流变性指标。结果:模型组家兔血浆尿素、肌酐在12 h、24 h、48 h均显著高于对照组,且随着时间延长组间呈升高趋势。模型组12 h红细胞电泳时间高于对照组,电泳率、电泳迁移率低于对照组;模型组24 h红细胞电泳时间低于12 h,电泳率、电泳迁移率显著高于12 h;模型组48 h红细胞电泳指标与其它各组均无统计学差异。模型组家兔血沉、血沉方程K值和血沉校正K值随时间延长呈升高趋势,但仅在48 h时显著高于对照组;各组间红细胞聚集指数、变形指数未见统计学差异。结论:在ARF发展进程中,红细胞电泳时间延长、电泳率与迁移率降低,后逐渐恢复正常;血沉指标逐渐增高。

Objective： To observe the change of erythrocyte/neology in rabbits with acute renal failure （ARF）. Methods： Thirty-eight healthy rabbits were randomly divided into control group （ n = 8）, model group （establishing ARF model da intramuscular injection 1% Hg- CI2, and divided into 12 h, 24 h, 48 h subgroups, all n = 10）, the arterial blood sample was taken out through carotid artery at corresponding times after arasthefization with urethane, for detecting the indices of renal function and erythmcyte/neology. Results： The levels of urea and creafinine in plasma of model rabbits at 12 h, 24 h and 48 h were higher than those of control group, and there was a rise trend along xcith the time extension. The erythrncytes electphoresis time at 12 h of model group was higher, the electrophoresis rate and migration rate of erythrocytes were lower compared with those of eontml group; the erythvytes electropberesis time at 24 h of model group was lower and the dectrophoresis rate and migration rate were higher compared with those of model group at 12 h; and there were no statistical differences in ehro- eytes electrophoresis indices between model group at 48 h and other groups. Meanwhile, there was a rise trencl in erythrncyte sedimentation rate （ESR）, K value of equation and emendation along with the time extension of ARF, but these indices only at 48 h of model group was lower significantly than that of control group. There were no statistical differences in aggregation index and deformability index of erythrocytes among groups. Conclusion： During the process of ARF, the erythrnoytes dectrophoresis time lengthen and electrophoresis rate and migration rate decrease at early stage, and these indices gradually return to normal; the indices of ESR increase gradually.