摘要
目的:评价反式玉米素(Trans-Zeatin,tZ)对HaCaT细胞的迁移和水通透性的影响。方法:RNA干扰实验;载有钙黄绿素的HaCaT细胞执行水的通透性测量。结果:反式玉米素缓解了UV导致的HaCaT细胞迁移能力的下降;并改善了HaCaT细胞由UV介导的水通透性的下降。结论:经Trans-Zeatin处理后可上调角蛋白细胞中小鼠水通道蛋白3(AQP3)的表达,促进HaCaT细胞的迁移和改善水的通透性。
Objective： To investigate the effect of trans - zeatin on the migration and water permeability of HaCaT cells. Methods： RNA interference （RNAi） experiments; Cells were loaded with calcein and water permeability mea- surement was performed. Results： tZ attenuates UV- induced down- regulation of migration in cultured skin kerati- nocytes （HaCaT cells）, tZ attenuates UV- induced downregulation of water permeability in cultured skin keratino- cytes （HaCaT cells）. Conclusion： we found that tZ treatment, which upregulates AQP3 expression, enhances cell migration and water permeability in keratinocytes.
出处
《中国麻风皮肤病杂志》
2013年第3期168-171,共4页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金项目(编号:810701297和81101188)
