NF-κB和VEGF在大鼠门静脉海绵样变性形成过程中的表达及意义

Expressions of NF-κB and VEGF in The Formation of Cavernous Transformation of Portal Vein in Rats

目的观察大鼠门静脉海绵样变性(CTPV)形成过程中,门静脉及周围组织核转录因子-κB(NF-κB)、血管内皮生长因子(VEGF)以及CD31的表达,并探讨NF-κB和VEGF与门静脉区血管新生的关系,及其在CTPV形成过程中的作用及意义。方法将110只SD大鼠随机(随机数字表法)分为模型组和假手术组。模型组大鼠采用21G口径的钝头针行门静脉部分缩窄术,建立CTPV动物模型;假手术组大鼠仅行门静脉游离探查。取假手术组和模型组大鼠门静脉及周围组织标本,用免疫组化染色方法检测NF-κB、VEGF及CD31的表达,通过Image Pro Plus 6.0软件测定NF-κB表达的光密度(OD)值和VEGF表达的平均积分光密度(IOD)值,并计算微血管密度(MVD)值。结果与术前相比,假手术组大鼠术后第1、2、3、4及6周NF-κB的核浆OD比值、VEGF的平均IOD值以及MVD值的差异均无统计学意义(P>0.05),而模型组大鼠术后各时间点的差异均具有统计学意义,术后各时间点较高(P<0.01)。术前假手术组大鼠NF-κB的核浆OD比值、VEGF的平均IOD值以及MVD值与模型组相比,差异均无统计学意义(P>0.05),而术后各时间点,模型组大鼠的上述3个指标与假手术组比较差异均具有统计学意义,模型组较高(P<0.01)。在模型组大鼠中,NF-κB的表达与VEGF的表达及MVD呈正相关(r=0.654 6,P<0.01;r=0.620 7,P<0.01),VEGF的表达与MVD也呈正相关(r=0.636 9,P<0.01)。结论 NF-κB及VEGF可能与CTPV的形成有关,并可能参与CTPV的血管新生。

Objective To observe the expression levels of nuclear factor kappa B（NF-κB）,vascular endothelial growth factor（VEGF）,and CD31 in portal vein and surrounding tissues of rats during the formation process of cavernous transformation of portal vein（CTPV）,and try to search the relationship between NF-κB,VEGF,and the angiogenesis of portal areas,as well as the significance and the role of NF-κB and VEGF in the formation process of CTPV.Methods One hundred and ten Sprague-Dawley（SD） rats were randomly（random number method） divided into sham operation group and model group.The partial constriction operations on portal vein were performed in model rats with a blunt 21G caliber to establish CTPV animal models（model group）,while the exploratory operations on portal vein,not constriction, were performed in rats of sham operation group.All specimens（portal vein and surrounding tissues） were fixed in formalin and made into paraffin blocks.Each specimen was tested by immunohistochemistry for the expressions of NF-κB,VEGF,and CD31,then optical density（OD） of NF-κB expression and the mean integral optical density（IOD） of VEGF expression were measured by using Image Pro Plus 6.0 software,and microvessel density（MVD） was calculated under microscope.Results Nucleoplasm ratio of OD value of NF-κB,mean IOD value of VEGF,and MVD value in 1,2,3,4,and 6 weeks after operation didn＇t significantly differed from that of before operation in sham operation group（P0.05）,but higher at all time points after operation in model group（P0.01）.Compared with sham operation group,nucleoplasm ratio of OD value of NF-κB,mean IOD value of VEGF,and MVD value were significantly higher in 1,2,3,4,and 6 weeks after operation in model group（P0.01）.NF-κB and VEGF,NF-κB and MVD,VEGF and MVD were positively correlated with each other（r=0.654 6,P0.01;r=0.620 7,P0.01;r=0.636 9,P0.01） in model group.Conclusion NF-κB and VEGF may relate to the formation of CTPV,and may involve in the angiogenesis.