期刊文献+

Id3上调E-cadherin表达抑制口腔鳞状细胞癌的侵袭 被引量:3

Expression of Id3 suppresses the invasiveness of human squamous cell carcinoma
下载PDF
导出
摘要 目的:研究Id3的表达在口腔鳞状细胞癌侵袭转移中的作用机制。方法:在UM-SCC-23口腔鳞状细胞癌中瞬时转染Id3真核表达载体,实时PCR和蛋白印迹法检测Id3和E-cadherin在基因和蛋白水平的表达。将E-cadherin启动子载体瞬时转染到UM-SCC-23细胞系中,利用荧光双报告基因系统检测Id3对E-cadherin启动子的调控作用。利用Id3稳定转染UM-SCC-23细胞系进行细胞侵袭实验。结果:Id3促进了E-cadherin基因和蛋白水平的表达,增强了E-cadherin启动子活性,稳定表达Id3的UM-SCC-23细胞系侵袭能力降低。结论:Id3能够促进E-cadherin表达,抑制口腔鳞状细胞癌的侵袭。 Objective: To investigate the role of Id3 in human squamous cell carcinoma.Methods: The Id3 expression vectors and control vectors were transiently transfected into UM-SCC-23 cells of human squamous cell carcinoma cell by Lipofectamine 2000TM,respectively. The mRNA and protein levels of Id3 and E-cadherin in UM-SCC-23 cells were detected by real-time polymerase chain reaction and Western blotting.UM-SCC-23 cells were transiently transfected with the E-cadherin promoter-reporter plasmid and we investigated the effect of Id3 on E-cadherin promoter activity in Dual Luciferase Reporter Assay System.Transwell cell migration assays were performed with Id3 stable cell lines.Results: Id3 up-regulated E-cadherin mRNA and protein levels.The Id3 enhanced the E-cadherin promoter activity. Id3 stable transfection UM-SCC-23 cells inhibited UM-SCC-23 cell invasion by cell invasion assay.Conclusion: This study suggests that Id3 increase the expression of E-cadherin and inhibit the invasion of human squamous cell carcinoma.
出处 《东南大学学报(医学版)》 CAS 2013年第2期154-157,共4页 Journal of Southeast University(Medical Science Edition)
基金 国家自然科学基金资助项目(30871390 30800415)
关键词 分化抑制因子3 E-CADHERIN 表达 侵袭转移 inhibitors of differentiation 3 E- cadherin expression invasion and metastasis
  • 相关文献

参考文献13

  • 1NORTON J D. ID helix-loop-helix proteins in cell growth,dif- ferentiation and tumorigenesis [ J]. J Cell Sei, 2000, 113 (22) :3897-3905.
  • 2PERK J, IAVARONE A, BENEZRA R. Id family of helix-loop- helix proteins in cancer [ J ]. Nat Rev Cancer, 2005,5 ( 8 ) : 603-614.
  • 3GUPTA G P,PERK J,ACHARYYA S,et al. ID genes mediate tumor reinitiation during breast cancer lung metastasis [ J ]. Proc Natl Acad Sci USA,2007,104(49) : 19506-19511.
  • 4NORTON J D, DEED R W, CRAGGS G, et al. Id helix-loop- helix proteins in cell growth and differentiation [ J 1. Trends Cell Biol, 1998,8 (2) :58-65.
  • 5ALANI R M,YOUNG A Z,SHIFFLETT C B. htl regulation of cellular senescence through transcriptional repression of p16/ Ink4a[ J]. Proc Natl Acad Sci ,2001,98(14) :7812-7816.
  • 6PRABHU S, IGNATOVA A ,PARK S T,et al. Regulation of the expression of cyclin-dependent kinase inhibitor p21 by E2A and Id proteins[ J]. Mol Cell Biol, 1997,17(10) :5888-5896.
  • 71 LASORELLA A,IAVARONE A,ISRAEL M A. ht2 specifically alters regulation of the cell cycle by tumor suppressor proteins [J]. Mol Cell Bio1,1996,16(6) :2570-2578.
  • 8COPPE J P,SMITH A P, DESPREZ P Y. Id proteins in epithe- lial cells[ J]. Exp Cell Res,2003,285( 1 ) :131-145.
  • 9LASORELLA A, UO T, IAVARONE A. Id proteins at the cross- road of development and cancer [ J ]. Oncogene,2001,20 (58) :8326-8333.
  • 10陈旭,汤玲玲,陈敏,等.Notch2存人恒牙牙髓及牙乳头巾表达研究[J].中闭实用口腔科杂志.2012,7(5):417-420.

二级参考文献5

共引文献3

同被引文献24

  • 1Chu H, Luo H, Wang H, et al. Silencing BMP-2 expression inhibits A549 and H460 cell proliferation and migration[J]. Diagn Pathol, 2014, 9:123.
  • 2Wang K, Feng H, Ren W, et a/. BMP9 inhibits the proliferation and invasiveness of breast of breast cancer cells MDA-MB-231[J].J Cancer Res C/in Oncol, 2011, 137(11)= 1687-1696.
  • 3Herrera B, van Dinther M, Ten DP, et al. Autocrine bone morphogenetic protein-9 signals through activin receptor-like kinase-2/Smadl/ Srnad4 to promote ovarian cancer cell proliferation[J]. Cancer Res, 2009, 69(24):9254-9262.
  • 4Ye L, Kynaston H, Jiang WG. Bone morphogenetic protein-9 induces apoptosis in prostate cancer cells, the role of prostate apoptosis response-4[J]. Mol Cancer Res, 2008, 6(10):1594-1606.
  • 5Li XJ, Zhu CD, Yu W, et al. Overexpression of Id3 induces apoptosis of A549 human lung adenocarcinoma cells[J]. Cell Pro/if,2012, 45(1 ):1-8.
  • 6Langenfeld EM, Calvano SE, Abou-Nukta F. The mature bone morphogenetic protein-2 is aberrantly expressed in non- small cell lung carcinomas and stimulates tumor growth of A549 cells[J]. Carcinogenesis, 2003, 24(9):1445-1454.
  • 7Kim M, Yoon S, Lee S, eta/. Gremlin-1 induces BMP-independent tumor cell proliferation, migration, and invasion[J]. PLoS One, 201 2, 7(4): e35100.
  • 8Leppiranta O, Tikkanen JM, Bespalov MM, et al. Bone morphogenetic protein- inducer tilorone identified by high- throughput screening is antifibrotic in vivo[j]. Am J Respir Cell Mol Biol, 2013, 48(4):448-455.
  • 9Genander M, Cook PJ, Ramsk61d D, et al. BMP signaling and its pSMAD1/5 target genes differentially regulate hair follicle stem cell lineages[J]. Cell Stern Cell, 2014, 15(5):619-633.
  • 10Richter J, Schlesner M, Hoffmann S, et al. Recurrent mutation of the ID3 gene in Burkitt lymphoma identified by integrated genome, exonle and transcriptome sequencing[J]. Nat Cenet, 2012, 44(12):1316-1320.

引证文献3

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部