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氦氖激光辐照致眼损伤的机制研究 被引量：3

Mechanism research of eye injury induced by He-Ne laser irradiation
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摘要 目的探讨氦氖激光致眼损伤效应及可能的作用机制。方法建立氦氖激光的大鼠眼损伤模型,通过组织病理染色、凋亡检测以及原位杂交等方法,观察激光照后视网膜细胞结构变化及谷氨酸受体(NMDAR)的表达。结果光学显微镜下观察显示激光照后视网膜出现组织结构的紊乱和感光细胞的凋亡,同时伴有NMDAR mRNA的高表达。结论氦氖激光在一定的条件下可造成视网膜的病理性损伤,损伤局部谷氨酸的过度释放及NMDAR受体的高表达可能是感光细胞受损的重要机制。 [Objective]To explore the mechanism of He-Ne laser on rat eyes damage.[Methods]The model of rat eyes damage by He-Ne laser was established.Through examination of histopathology,TUNEL and in situ hybridization（ISH）,the expression of glutamate receptor（NMDAR） and changes in retinal cell structure were observed.[Results] Observation under the optical microscope showed disorders of the organizational structure,apoptosis of photoreceptor cells and increased expression of NMDAR in retina after exposure to laser.[Conclusion]Under given conditions,He-Ne laser irradiation can cause retina pathological changes,the excessive release of glutamateand and high expressions on NMDAR may be the possible mechanism of damage to the photoreceptor cells.
作者 梁洁 陈鹏 闫文华 马萍 方毅 康宏向 高荣莲 王长振
机构地区 中国人民解放军军事医学科学院放射与辐射医学研究所电磁与激光生物学研究室 军事医学科学院附属
出处 《职业与健康》 CAS 2013年第6期641-643,共3页 Occupation and Health
基金 国家自然科学基金(项目编号:81072239 项目编号:81000685)
关键词 激光 视网膜 损伤 NMDAR 机制 Laser Retina Injury NMDAR mechanism
分类号 R135.92 [医药卫生—劳动卫生]
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参考文献9

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二级参考文献11

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共引文献13

同被引文献22

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引证文献3

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职业与健康
2013年 第6期

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