129株志贺菌血清分型及耐药性分析

Serotype distribution and drug resistance of 129 Shigella strains

目的调查上海市闵行区2008—2010年志贺菌流行的主要血清型和耐药情况,为细菌性痢疾预防和临床用药提供依据。方法收集2008—2010年闵行区肠道门诊监测点腹泻患者肛拭样本中分离到的129株志贺菌进行生化鉴定、血清学分型,并用K-B法测定其对10种抗生素的药物敏感情况。结果 129株志贺菌中福氏志贺菌89株,占69.00%,其中F2a型占74.16%;宋内氏志贺菌40株,占31.00%;未分离出其他群型的志贺菌。药敏结果显示,129株志贺菌对四环素、复方新诺明、氨苄西林、利福平和萘啶酸耐药显著,均大于80%;对环丙沙星、阿莫西林比较敏感。但福氏志贺菌与宋内氏志贺菌之间差异有统计学意义;多重耐药率达92.24%以上,以6种耐药为主。结论该地区2008—2010年志贺菌流行菌型为F2a,福氏志贺氏菌和宋内氏志贺菌对环丙沙星、阿莫西林敏感,对复方新诺明、氨苄西林、四环素、萘啶酸、利福平等多种抗菌药物耐药显著,多重耐药现象日趋严重。应加强耐药性监测。

[Objective]To investigate the stereotyping and antibiotic-susceptibility of Shigella in Minhang District of Shanghai City from 2008 to 2010,and provide evidence for bacillary dysentery prevention and clinical treatment.[Methods]Rectal swab specimens of patients with acute diarrhea in intestinal outpatient monitoring points of Minghang District from 2008-2010 were collected for isolation of 129 strains of Shigella by using biochemical test and serum agglutination test.KB method was used for testing antibiotic-susceptibility of Shigella.[Results] A total of 129 strains of Shigella were isolated,among which flexneri Shigella bacteria accounted for 69.00%,Sonnei Shigella bacteria accounted for 31.00%.The rate of resistance to tetracycline,cotrimoxazole,ampicillin,rifampicin and nalidixic acid was significantly higher than 80% respectively.Otherwise,these isolates was sensitive to ciprofloxacin,amoxicillin.The significance of difference existed between flexneri Shigella and Sonnei Shigella.Multi-drug resistance rate of 129 strains of Shigella was 92.24%,mainly to 6 kinds of antibiotics.[Conclusion]The predominant serotype was F2a in Minhang District from 2008 to 2010.The resistance of Flexneri Shigella sonnei Shigella to ciprofloxacin,amoxicillin,cotrimoxazole,ampicillin,tetracycline,rifampin,nalidixic acid is significant.Multi-drug resistant phenomenon is worsening.The surveillance of antimicrobial resistance of Shigella should be strengthened.