摘要
目的:优化CCK-8试剂盒在血液毒理学检测中的应用条件。方法:以CCK-8试剂盒标准检测法和两种优化的方法分别检测不同浓度的氢醌(hydroquinone,HQ)对TK6细胞增殖速度的影响,分别与计数法比较,分析影响实验检测的原因。结果:试剂盒标准方法中,HQ对CCK-8试剂的检测结果有影响,结果出现偏差,优化后的两种方法与计数法检测结果较为相似。结论:HQ对CCK-8试剂检测结果有影响,优化出一种适用于HQ血液毒理学检测的细胞增殖方法。
Objective：To optimize the applicable condition for CCK -8 cell viability kit in hematotoxicology research. Methods： The CCK- 8 reagent kit and two optimized methods were used respectively to observe the influence of hydroquinone with different concentrations on proliferation of TK6 ceils, then the results were compared with that by cell counting method to analyze the influencing factors. Results： Detection results based on the protocol demonstrated that HQ influenced the CCK - 8 reagent function, and led to discrepancy compared to the cell counting method, while results from the two optimized methods showed similarity with the cell counting method. Conclusion： HQ can influence the results with CCK - 8 reagent, so a detection method of cell proliferation was optimized in hematotoxicology for this kit.
出处
《中国卫生检验杂志》
北大核心
2013年第3期628-630,共3页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金资助(81273116)
东莞市科技计划项目(201010815206)
广东医学院医学科研基金(M2011005)
关键词
血液毒理学
氢醌
TK6细胞
细胞增殖
Hematotoxicology
Hydroquinone＇
TK6 cell
Cell proliferation
