流感病毒检测能力考核结果分析

Assessment of influenza virus testing capability

目的:通过对流感病毒核酸考核盲样进行检测,及时找出问题,以确立简便、快速、特异的检测技术。方法:用RT-PCR法对10份考核盲样进行了检测,对不能确定型别的盲样用Real-time PCR法重新检测。结果:根据国家流感中心的反馈结果,RT-PCR法检测10份盲样9份结果正确,3号盲样用RT-PCR法检测新甲型H1N1和人季节性H1均为阴性,用Real-time PCR法复检为新甲型H1N1阳性。结论:Real-time PCR法具有灵敏度高、特异性好、重复性好等优点,适用于流感疫情的快速诊断。

Objective：By detecting the blind samples of influenza virus nucleic acid, to identify the problems timely, so as to establish the simple, fast and specific test technology. Methods： Using RT - PCR to detect ten tes- ting blind samples, the unidentified samples were detected by real - time PCR method to re - test. Results ： Accord- ing to feedback from the results of the Chinese National Influenza Center（ CNIC）, nine of the ten blind samples were detected with correct results using RT -PCR. While NO. 3 blind sample was detected negative for new HIN1 and seasonal H1 by RT - PCR and by real - time PCR method for reexamination, it was found new H1 N1 influenza virus nucleic acid positive. Conclusion： Real -time PCR method with high sensitivity, high specificity and good reproducibility is suitable for rapid influenza diagnosis.