实时荧光PCR技术在手足口病检测中的应用

Application of real-time fluorescent quantitative RT-PCR assay in the hand-foot-mouth disease detection

目的:对手足口病患者标本进行病毒核酸检测,探讨实时荧光定量RT-PCR方法在手足口病检测中的意义。方法:用实时荧光定量RT-PCR方法对2011年度淮北市采集的257份标本进行肠道病毒核酸检测,并对肠道病毒(EV71)、柯萨奇病毒A组16型(CoxA16)进行分型;同时,与常规RT-PCR的方法进行了比对。结果:257份标本中肠道病毒核酸阳性138份,阳性率为53.70%,其中,EV71阳性标本89份,阳性率34.63%,CoxA16型核酸阳性34份,阳性率13.23,未分型15份,占病例总数的5.84%。结论:实时荧光定量RT-PCR方法耗时短、特异性强、灵敏度高,可作为手足口病检测的诊断方法。

Objective：To apply a method of real - time fluorescent quantitative RT - PCR for detecting samples from patients, and to examine its utility in diagnosis of hand - food - mouth disease （HFMD）. Methods ： A total of 257 samples from HFMD patients in Huaibei were collected in 2011, and they were detected by real - time fluores- cent quantitative PCR method. The samples were subjected to screen in population of enterovirus with enterovinus 71 （EV71） and Coxasckievirus A 16 （CoxA16） ,and the results were compared with those by conventional RT- PCR. Results ： One hundred and thirty - eight samples were found intestinal virus nucleic acid positive in 257 specimens, the positive rate was 53.70%, of which, eighty - nine samples were EVT1 positive, the positive rate was 34.63%, thirty ,four samples were CoxA16 positive, the positive rate was 13.23%, while 15 samples were untyped, ac- counting for 5.84% of the total cases. Conclusion ： Real - time fluorescent quantitative PCR method has short time consumption, strong specificity, high sensitivity, can be used as a method for diagnosis of foot and mouth disease.