782株分枝杆菌培养结果分析与流行病学特点

Analysis of culture result and epidemiological features of 782 mycobacteria strains

目的:回顾性分析我院2010年间分枝杆菌培养阳性资料。方法:送检标本经BACTEC MGIT 960和罗氏培养,对阳性标本进行菌型鉴定和PLWHA感染病人耐药性分析。结果:BACTEC MGIT 960与罗氏培养在分枝杆菌检出率方面有显著统计学差异(P<0.001);送检和阳性检出标本比较显示,结核分枝杆菌感染在性别分布中无统计学差异(P>0.05)。85%(17/20)PLWHA感染病人对一线抗结核药物敏感。结论:(1)BACTEC MGIT 960阳性检出率高于罗氏培养,所用培养时间比罗氏短。(2)结核分枝杆菌的感染无性别倾向性,19岁～44岁是发病率较高的年龄段。(3)PLWHA感染病人对结核分枝杆菌感染耐药率不高。

Objective：To retrospectively analyze the mycobacteria culturepositive specimens in 2010 in Kunming Third People＇s Hospital. Methods： The specimens were cultured via BACTEC MGIT 960 and Lowenstein- Jensen medium, then the positive cases were carried out bacteria identification and drug resistance analysis for PL- WHA. Results： There was significant statistical difference in detection rates of mycobacteria with BACTEC MGIT 960 and Lowenstein -Jensen medium（ P 〈0. 001 ）, but mycobacterium tuberculosis infection cases showed no statis- tical difference between genders （P 〉 0.05 ）. Among 20 PLWHA patients, seventeen cases were sensitive to first - line anti - tuberculosis drugs （85%）. Conclusion： 1. BACTEC MGIT 960 had higher positive rate and less cuhure time than the Lowenstein - Jensen medium. 2. The mycobacterium tuberculosis infection had no gender tendency and occurred frequently in people of 19 -44 years old, 3. The PLWHA were lower resistant to mycobacterium tuberculo- S/,S.