妊娠期高血压疾病大鼠甲状腺超微结构和功能变化的联系

The contact between thyroid ultrastructure and function change with hypertensive disorders in pregnancy in wistar rats

目的研究妊娠期高血压疾病大鼠甲状腺激素水平及甲状腺组织的超微病理改变,探讨妊娠期高血压疾病甲状腺组织结构损伤是否与其激素水平变化相一致。方法采用亚硝基左旋精氨酸甲酯(L-NAME)建立妊娠期高血压疾病模型大鼠,发光免疫法测定正常妊娠组、妊娠期高血压疾病模型组(PIH组)大鼠血清中FT3、FT4及TSH的浓度变化,透射电镜下观察甲状腺组织的结构变化。结果 PIH组FT3、FT4水平较正常妊娠组呈下降趋势,TSH水平显著升高(P<0.05);电镜下观察PIH组大鼠甲状腺滤泡上皮细胞损伤明显,主要显示甲状腺功能低下。结论甲状腺亦是妊娠期高血压疾病慢性攻击的靶器官,妊娠期高血压疾病存在甲状腺组织形态学的改变,与血清中激素浓度变化意义基本相同。

Objective： To investigate thyroid hormone levels and the ultrastructural changes of thyroid tissues with hypertensive disorders in pregnancy in wistar rats and to discuss whether thytoid ussue structural damage is consistent with hormone level changes. Methods： Rat model of hypertensive disorders in pregnancy was established by L- nitro- arginine methyl easter （L- NAME）, lumi- nescence immunoassay method for the determination of the normal pregnancy group, model group of hypertensive disorders in pregnancy （PIH group） rats serum FT3, FT4 and TSH concentration change, and observe the ultrastructural changes of thyroid tissue by transmission electron microscope. Results ： The PIH group of FT3, FT4 than normal pregnancy group had a descending trend, TSH level increased significantly （ P 〈 0. 05 ） ; By electron microscopy the PIH group of thyroid follicular epithelial cells damage obvious, mainly display thyroid dysfunction. Conclusion： The thyroid is also a target organ of chronic attack of hypertensive disorders in pregnancy, hypertensive disorders in pregnancy occurred basically the same as the change in the morphology of the thyroid tissue as serum hormone concentrations significance.