红曲色素高产菌株的优选及其发酵条件优化

Screen of monascus pigment high-yeilding strain and optimation of culture condition

对实验室保藏的红曲霉菌株进行了优选,获得了产量较高的红曲色素生产菌株Monascus purpureus M5,其液态发酵的红色素产量达到了154.5U/mL。对M5发酵产色素的碳源、氮源、初始pH值等进行了优化,以70g/L大米粉、30g/L可溶性淀粉、10g/L黄豆粉、1g/L NaNO3为发酵培养基,初始pH值4.0,35℃条件下培养6d,M5摇瓶发酵红色素的产量达到195.8U/mL。在5L发酵罐中对M5进行葡萄糖补料分批发酵培养,其色素产量在108h时达到最大值253.2U/mL,相比摇瓶分批发酵提高了29.3%,成效显著。

Through strain screening, a monacus pigment high-yielding strain Monascus purpureus M5 was obtained. In shaking flask scaled fermenta- tion, the yield of red pigment was 154.5U/ml. The effects of carbon sources, nitrogen sources and pH value on the production of monascus pigment were studied. The highest yield of monacus pigment （195.8U/ml） appeared as the following conditions： rice powder 70.0g/L, soluble starch 30.0g/L, soybean powder 10.0g/L and NaNO3 1.0g/L, temperature 35℃and the initial pH value 4.0. In 5L ferrnentor, the highest yield of red pigment reached 253.2U/ml at 108h when glucose was fed, which increased 29.3% compared with the batch fermentation （195.8U/ml）.