孔石莼多糖对糖尿病小鼠抗氧化能力的影响

Effects of polysaccharide from Ulva pertusa Kjellm on antioxidant capacity in diabetic mice

目的:研究孔石莼多糖抗氧化活性。方法:采用传统的水提醇沉法提取孔石莼多糖,以四氧嘧啶诱导的糖尿病小鼠为模型,分为孔石莼多糖低、高剂量组、阳性对照组、模型组及正常组。分别以不同剂量孔石莼多糖(250mg/kg、1000mg/kg)、二甲双胍(1000mg/kg)和蒸馏水灌胃,28d后测定小鼠肝脏和肾脏组织中SOD、GSH-Px、CAT活性和MDA含量。结果:高剂量孔石莼多糖能显著提高糖尿病小鼠肝脏和肾脏组织中SOD、GSH-Px和CAT的活性,降低MDA含量。结论:孔石莼多糖具有显著的抗氧化作用。

Purpose： To study the antioxidant capacity of polysaccharide extracted from Ulva pertusa Kjellm. Methods： The polysaccharide from Ulva pertusa Kjellm was extracted with hot water and precipitated with ethanol. The diabetic mice was established by intraperitoneal injection of alloxan with a dose of 200mg/kg body weight. The diabetic mice were randomly divided into four groups： low and high dosage ofpolysaccharide （250mg/kg and 1000mg/kg body weight）, positive control group （metformin at 1000mg/kg body weight） and diabetic model group. The normal mice were use as normal control group. After 28d, the activities of SOD, GSH-Px and CAT and the content of MDA in liver and kidney were determined. Results： The polysaccharide-treated mice exhibited significantly higher activities of SOD, GSH-Px and CAT in liver and kidney and significantly lower content of MDA as compared with diabetic model group. Conclusion： It suggested that the polysaccharide from Ulva pertusa Kjellm have strong antioxidant a- bility to protect against alloxan-induced oxidative damage.