应用流式细胞术检测EphA2及其调节基因HoxA1、C-myc在喉癌组织中的表达及相互关系

THE EXPRESSION AND RELATIONS OF EPHA2,HoxA1 AND C-myc IN LARYNGEAL CARCINOMA WITH FLOW CYTOMETERE

目的从蛋白水平探讨HoxA1、C-myc、EphA2与喉癌发生发展的关系,从分子生物学角度探讨喉癌的发病机制。方法①喉癌组织40例及癌旁组织40例,取15例非喉癌患者的喉部正常黏膜组织做对照。所有肿瘤组织均经病理学确诊为鳞状细胞癌,癌旁组织及喉部正常黏膜均经病理证实为炎症或正常黏膜。②应用流式细胞术检测喉癌组织、癌旁组织及喉部正常黏膜组织的EphA2和HoxA1、C-myc蛋白表达的含量。结果 EphA2蛋白、HoxA1蛋白、C-myc蛋白在喉癌组织中的表达量分别高于癌旁组织和正常喉黏膜组织,而癌旁组织与正常黏膜组织间的表达差异无统计学意义。喉癌组织中EphA2蛋白与HoxA1蛋白、C-myc蛋白有多元线性回归关系,EphA2蛋白与HoxA1蛋白呈正相关,与C-myc蛋白呈负相关,Y=0.983+0.739X1-0.323X_2(P=0.000)。结论 EphA2蛋白、HoxA1蛋白和C-myc蛋白在喉癌中的表达与喉癌的发生发展有关。

Objective To explore the genesis mechanism of laryngeal carcinoma at the level of molecular biology. Methods （1）Forty laryngeal carcinoma fresh samples were analyzed and meanwhile 40 para-carcinoma tissues and 15 normal laryngeal mucosa samples were also studied as controls. All tumor tissues were confirmed to be squamous-cell carcinoma; the para-carcinoma tissues and normal mucosa were confirmed to be inflammatory or normal mucosa pathologically. （2）The expression of erythropoietin- producing hepatocellular receptor A2 （ EphA2 ）, Hox genes A1 （ HoxA1 ） and cell-myc （ C-myc ） protein were measured with flow cytometere. Fluorescence index was defined as the quantitative expression index of EphA2, HoxA1 and C-myc protein. Results The quantitative and qualitative expressions of EphA2 protein, HoxA1 protein, C-myc protein in laryngeal carcinoma tissues were obviously higher than those in para-carcinoma and in normal laryngeal mucosa tissues respectively; There was no significant difference between the expression of para-carcinoma and normal laryngeal mucosa tissues. There was multiple linear regression relationship in the expression of EphA2, HoxA1 and C-myc in laryngeal carcinoma. The expression of EphA2 protein was positively correlated with HoxA1 protein, while negatively correlated with C-myc protein. The multiple linear regression equation was Y = 0. 983 ＋ 0. 739X1 - 0. 323X2 （ P = 0. 000 ） o Conclusion The expression of EphA2, HoxA1 and C-myc contribute to the carcinogenesis anddevelopment of laryngeal carcinoma.