高效氯氰菊酯对草鱼器官酸性磷酸酶活性的影响

Effects of Beta-cypermethrin on Acid Phosphatase Activity in Organs of Grass Carp

研究了暴露于不同高效氯氰菊酯浓度下草鱼Ctenopharyngodon idellas鳃、肝胰脏和肾脏的酸性磷酸酶(Acid Phosphatase,ACP)的活性变化。实验中高效氯氰菊酯浓度设5组,其中对照组1个(0μg/L),处理组4个(0.5μg/L、1.0μg/L、3.0μg/L、5.0μg/L)。每组随机投放40尾草鱼,分别于1d、5d、12d取样,测定鳃、肝胰脏和肾脏的ACP活性。结果显示,鳃ACP活性低浓度(0.5μg/L、1.0μg/L)短时间(1d)暴露下显著增加(P<0.01),高浓度(3.0μg/L、5.0μg/L)暴露时显著下降(P<0.01);肝胰脏ACP活性暴露1d时,除0.5μg/L组外,其他处理组均显著下降(P<0.05、P<0.01),暴露5d、12d时,各处理组均显著上升(P<0.05、P<0.01);肾脏ACP活性除0.5μg/L组在暴露1d、5d时无显著变化(P>0.05)外,其他处理组在暴露时间内均显著下降(P<0.01)。实验表明:高效氯氰菊酯能够通过影响草鱼鳃、肝胰脏和肾脏中酶的活性,干扰这些器官的代谢平衡,进而对这些器官产生毒害作用。

The change on the activity of acid phosphatase（ACP） in gill,hepatopancreas and kidney of grass carp（Ctenopharyngodon idellas） exposed to different concentration of beta-cypermethrin was researched.In this study,grass carps were randomly divided into five groups（each group with 40 fish） including a control group（0 μg/L） and four different beta-cypermethrin concentration treatment groups（0.5 μg/L,1.0 μg/L,3.0 μg/L,5.0 μg/L）.The sample from the first day,fifth day and twelfth day of each group were utilized to determine the activity of ACP in gill,hepatopancreas and kidney.The results showed that ACP activity in the gill at 0.5 μg/L,1.0 μg/L significantly increased（P0.01） when exposed in the first day,whereas reduced activity（P0.01） were recorded at 3.0 μg/L and 5.0 μg/L.ACP activity in hepatopancreas at the other treatment groups except 0.5 μg/L group decreased remarkably（P0.05,P0.01） in the first day,whereas ACP activity significantly increased（P0.05,P0.01） for all treatment groups when exposure duration last to fifth day and twelfth day.In the case of kidney,ACP activity was reduced remarkably（P0.01） for all treatment groups except 0.5 μg/L group in the first day and fifth day.In conclusion,beta-cypermethrin disrupted the metabolic balance of these organs by affecting grass carp gill,hepatopancreas and kidney enzyme activity,thus produce toxic effects to these organs.