摘要
The aim of this study was to isolate the chemical constituents of the aerial parts of Polygala tenuifolia Willd. and to determine their antioxidant activities. Ten flavonoids were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. Their structures were elucidated by spectroscopic analysis and identified as isorhamnetin-3-O-13-D-glucopyranoside （1）, isorhamnetin-3-O-13-D-galactopyranoside （2）, quercetin-3-O-13-o-glucopyranosyl （1--＊2）-I^-D-galactopyranoside （3）, quercetin-3-O-13-O-glucopyranosyl （1---~2）-13-D-glucopyranoside （4）, linarin （5）, quercetin-3-O- 13-D-glucopyranoside （6）, 5,7-dihydroxy-8-methxoyflavone-7-O-13-o-glucuronoside （7）, isorhamnetin （8）, kaempferol （9） and quercetin （10）. All these compounds were isolated from this plant for the first time, and compounds 1-5 and 7 were isolated from the genus of Polygala for the first time. The antioxidant activities of the isolated compounds were evaluated by DPPH free radical scavenging assay, and compounds 3, 4, 6, 8, 9 and 10 showed potent antioxidant activities.
研究远志地上部分的化学成分并测试其抗氧化活性。运用硅胶、Sephadex LH-20、ODS及半制备液相,从远志地上部分中分离得到10个黄酮类化合物。用DPPH自由基清除试验测定了其抗氧化活性。分离所得化合物经光谱数据分析鉴定其结构分别为:异鼠李素-3-O-β-D-吡喃葡萄糖苷(1),异鼠李素-3-O-β-D-吡喃半乳糖苷(2),槲皮素-3-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃半乳糖苷(3),槲皮素-3-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃葡萄糖苷(4),蒙花苷(5),槲皮素-3-O-β-D-吡喃葡萄糖苷(6),5,7-二羟基-3-甲氧基黄酮-7-O-β-D-葡萄糖醛酸(7),异鼠李素(8),山奈酚(9)和槲皮素(10)。所有化合物均为首次从该植物中分离得到,化合物1–5和7均为首次从该属植物中分离得到。活性测试结果表明,化合物3、4、6、8、9和10表现出一定的抗氧化活性。
基金
New-Century Talent Program, Ministry of Education of China (Grant No.985-2-102-113)
National Science Fund for Excellent Young Scholars (Grant No. 81222051)
National Key Technology R&D Program "New Drug Innovation" of China (Grant No. 2012ZX09304-005, 2012ZX09301002-002)
