高效液相色谱法测定甘芍颗粒中甘草苷和甘草酸的含量

Determination of liquiritin and glycyrrhizic acid in Ganshao granules by HPLC

目的建立甘芍颗粒中甘草苷和甘草酸的含量测定方法。方法色谱柱为KromasilC18色谱柱(250mm×4.6mm,5μm),流动相为乙腈和磷酸盐溶液,梯度洗脱,流速为1 mL min-1,柱温为25℃,检测波长为237 nm,样品用甲醇超声提取后进样分析。结果甘草苷在0.02～0.18μg与峰面积线性关系良好(r=0.9997),甘草酸铵在0.02～0.4μg(甘草酸在0.019 6～0.391 9μg,甘草酸量=甘草酸铵重量/1.020 7)与峰面积线性关系良好(r=1.000 0);平均回收率101.8%。结论本方法简便、准确、灵敏度高、重复性好,可用于甘芍颗粒中甘草苷和甘草酸铵的含量测定。

Objective To establish a quality control method for glycyrrhizinate acid and liquiritin in Ganshao granules.Methods The column was Kromasil C18（250 mm×4.6 mm,5 μm） with the mobile phase composed of acetonitrile and phosphate buffer solution.The flow rate was 1 mL min-1;gradient elution.The column temperature was 25 ℃.The detection wavelength was 237 nm.The sample was analysed.Results Glycyrrhizinate acid showed excellent regression（r = 0.999 7） at 0.02-0.18 μg;ammonium glycyrrhizinate showed excellent regression（r = 1.000 0） at 0.02-0.4 μg [glycyrrhizic acid content was 0.019 6-0.391 9 μg,glycyrrhizic acid content =（ammonium glycyrrhizinate content/1.020 7].The average recovery was 101.8%.Conclusion The method is simple,accurate,sensible and reproducible,which can be used to control the quality of liquiritin and ammonium glycyrrhizinate.