茶叶梗固态发酵产单宁酶的响应面法优化

Optimization of Tannase Production with Tea Stalks Under Solid State Fermentation Using Response Surface Methodology

为获得茶叶梗固态发酵产单宁酶的最佳发酵条件,在单因素试验基础上,通过响应面法结合中心组合设计建立茶叶梗固态发酵产单宁酶的二次多项数学模型,探讨主要因素的影响效应及其交互作用。试验结果表明对单宁酶活力的影响:NH_4Cl添加量大于盐溶液pH。优化出的最佳条件是:NH_4Cl添加量5.69%、盐溶液pH6.06,在此条件下酶活达到56.7U/gds(全称为U/g dry substrate,即每克干基质对应的酶活)。验证试验获得的试验值与模型的预测值(55.58U/gds)基本相符,表明用响应面建立的模型适合于茶叶梗固态发酵工艺。

In this paper, solid state fermentation condition was optimized to enhance tannase production with tea stalks. Based on the results from single factor tests, response surface methodology（RSM） combined with central composite design was employed. A second order quadratic equation for tannase production was built to study the effect of main factors and their corresponding relationships. The results indicated that the effect of NH4C1 content on tannase activity is more than that of salt solution＇s pH. Under the optimal condition （5.69% NH4C1 and salt solution＇s pH 6.06）, tannase activity could reach 56.7 U/gds. This experimental result was in good agreement with the predicted value （55.58 U/gds） according to response surface analysis.