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肿瘤靶向性聚酰胺-胺型树枝状高分子基因载体的合成及性能研究 被引量：2

Synthesis and Characterization of Polyamidoamine Dendrimers Conjugating Transferrin as Tumor-targeted Gene Carriers
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摘要 通过二硫键可逆地将转铁蛋白偶联在以季戊四醇衍生物为核的第5代聚酰胺-胺型树枝状大分子(G5PDPAMAM)载体表面上,合成出肿瘤靶向性基因载体G5PDPAMAM-dsTf.系统研究了该载体介导绿色荧光蛋白质粒pEFGP-C1转染不同细胞的性能.结果表明,相比于G5PD PAMAM对癌细胞MCF-7和HepG2的转染效率,G5PDPAMAM-dsTf的转染性能均有不同程度的提高,显示出较好的肿瘤靶向性.同时,游离转铁蛋白的拮抗实验也证明了G5PD PAMAM-dsTf可以通过癌细胞表面的转铁蛋白受体TFR介导进入细胞. In an attempt to investigate the effect of transferrin conjugation on the transfection ability of the G5 PD PAMAM/DNA complexes,a tumor-targeted gene carriers,indicated as G5 PD PAMAM-Tf was synthesized by conjugating transferrin via reversible disulfide linkage on PAMAM surface.The important parameters for the physico-chemical properties of this delivery system were investigated and the transfection efficiencies of G5 PD PAMAM-Tf were also evaluated in different cell lines.The results showed that transferrin modification could enhance the delivery of pEGFP-C1 into tumor cells MCF-7 and HepG2.Meanwhile,the addition of free transferrin could significantly decrease the transfection efficiencies of G5 PD PAMAM-Tf in tumor cells,which indicated that the cellular uptake of such vectors is mainly based on transferrin receptor-mediated endocytosis.
作者 石楠 张悦 王燕铭
机构地区 南开大学药学院 南开大学化学科学学院
出处 《南开大学学报（自然科学版）》 CAS CSCD 北大核心 2012年第6期7-11,共5页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家自然科学基金(50803029)
关键词 聚酰胺-胺型(PAMAM)高分子 非病毒基因载体 转铁蛋白 肿瘤靶向性 polyamidoamine dendrimers non-vorial gene vectors transferrin tumor-targeted
分类号 Q819 [生物学—生物工程]
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参考文献11

  • 1Edelstein M L, Abedi M R, Wixon J. Gene therapy clinical trials worldwide to 2007-An update[J]. J Gene Med, 2007, 9: 833-842.
  • 2Korpisalo P, Yla-Herttuala S. Stimulation of functional vessel growth by gene therapy[J]. Integr Biol, 2010, 2:102 -112.
  • 3Pathak A, Kumar P, Chuttani K, et al. Gene expression, biodistribution, and pharmacoscintigraphic evaluation of chondroitin sulfate-PEI nanoconstructs mediated tumor gene therapy[J]. ACS Nano, 2009, 3(9): 1 493-1 505.
  • 4Droumaguet Le B, Nicolas J. Recent advances in the design of bioconjugates from controlled/living radical polymer- ization[J]. Polym Chem, 2010, 1: 563-598.
  • 5Kuo J S, Liou M, Chiu H. Evaluating the gene-expression profiles of HeLa cancer cells treated with activated and nonactivated poly(amidoamine) dendrimers, and their DNA complexes[J]. Mol Pharmaceutics, 2010, 7 (3): 805-814.
  • 6Allen T M. Ligand-targeted therapeutics in anticancer therapy [J]. Nature Reviews, 2002, 2(10):750-763.
  • 7Wang Y, Kong W, Song Y, el at. Polyamidoamine dendrimers with a modified pentaerythritol core having high effi- ciency and low cytotoxicity as gene carriers [J]. Biomacromolecules, 2009, 10(3) : 617- 622.
  • 8Roulon T, Helene C, Escude C. Coupling of a targeting peptide to plasmid DNA using a new type of padlock oligonucleotide[J]. Bioconjugate Chem, 2002, 13(5): 1 134-1 139.
  • 9Daniel W P, David P, Robert L. Design of imidazole-containing endosomolytic biopolymers for gene delivery[J]. Biotechnol Bioeng, 2000, 67(2): 217-223.
  • 10Mao H Q, Roy K, Troung-Le V L, et al. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficienc[J]. J Control Rel, 2001, 70 : 399- 421.

同被引文献46

  • 1赖人旭,张世能,Toshikazu Nakamura,袁世珍.NK4基因转染对裸鼠胰腺癌移植瘤生长的影响[J].癌症,2005,24(10):1191-1195. 被引量：5
  • 2BRAECKMANS K, BUYENS K, NAEYE B, et al. Advanced fluores-cence microscopy methods illuminate the transfection pathway of nucleic acid nanoparticles [ J ]. J Control Release, 2010,148( 1 ) :69-71.
  • 3QUADIR M A, HAAG R. Biofunetional nanosystems based on dendritic polymers [ J ].J Control Release, 2012,161 (2) :484.
  • 4TOMALIA D A, BAKER H, DEWALD J, et al.A newclass of polymers : starburst-dendritic macromolecules [ J ]. Polym, 1985, 17( 1 ) : 117-132.
  • 5SASAKI E, SUEMIZU H, SHIMADA A, et al. Generation of transgenic non-human primates with gennline transmission [ J ]. Nature, 2009,459 ( 7246 ) : 523 -527.
  • 6BAKER J R .Dendrimer-based nanoparticles for cancer therapy [ J] .Hematol Soc Hematol Educ Program,2009( 1 ):708-719.
  • 7SHARMA A, GAUTAM S P, GUPTA A K. Surface modified dendrimers: synthesis and characterization for Cancer targeted drug delivery [ J l-Bioorg Med Chem, 2011,19 ( 11 ) : 3341-3346.
  • 8MUKHERJEE S P, LYNG F M, GATCIA A, et al. Mechanistic studies of in vitro cytotoxicity of poly (amidoamine) dendrimers in mammalian ceils [ J ]. Toxicol Appl Pharmacol, 2010, 248 (3) :259-268.
  • 9PARIMI S, BARNES T J, CALLEN D F, et al. Mechanistic insight into cell growth, Interna-lization, and cytotoxicty of PAMAM dendrimers [ J ]. Biomacromolecules, 2010, 11 ( 2 ) : 382-389.
  • 10XIANG S N, TONG H J, SHI Q, et al. Uptake mechanisms of non- viral gene delivery [ J ]. J Control Release, 2011,158 ( 3 ) : 371.

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南开大学学报（自然科学版）
2012年 第6期

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