膀胱尿路上皮癌及正常膀胱组织microRNA21、205表达研究

Expression of miR-21 and miR-205 in urothelial cell carcinoma of bladder and normal bladder mucosa

目的探讨microRNAs(miRNAs)在膀胱尿路上皮癌与正常膀胱组织中的表达差异性及意义。方法采用基于2-△△CT的实时定量PCR(Real-time PCR)方法检测50例膀胱尿路上皮癌与21例正常膀胱组织中miR-21及miR-205的表达情况。结果与正常组织相比,癌组织miR-21表达上调(P<0.01),miR-205表达下调(P<0.01)。癌组织中miR-21与miR-205表达量的比值约3.6倍于正常组织中表达量的比值。结论 miR-21高表达、miR-205低表达可能参与膀胱尿路上皮癌的发生、发展。

Objective To detect the expression of miR-21 and miR-205 in urothelial cell carcinoma of bladder and normal bladder mucosa and explore the relationship between mieroRNAs （miRNAs） and urothelial cell carcinoma of blad- der. Methods 2cT Real-time PCR method was used for quantitative analysis of the expression of miR-21 and miR-205 in 50 urothelial cell carcinoma of bladder and 21 normal bladder tissues. Results Compared with normal bladder mucosa, the urothelial cell carcinoma of bladder tissues showed significant miR-21 over-expression （P 〈 0.01 ） and miR-205 down- regulation （ P 〈 0.01 ）. Cancer tissue showed miR-21 ： miR-205 ratio at least 3.6-fold higher than the quantitative ratio ob- tained from normal bladder tissue. Conclusion The over expression of miR-21 and lower expression of miR-205 may par- ticipate in recurrence urothelial cell carcinoma of bladder.