期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

2型糖尿病患者脂肪组织中的HO-1表达及临床意义 被引量：2

Expression and significance of hemeoxygenase-1(HO-1) in subcutaneous and omental adipose tissue of type 2 diabetic patients
下载PDF
导出
摘要 目的探讨2型糖尿病(T2DM)患者脂肪组织中血红素加氧酶-1(HO-1)基因及蛋白表达的临床意义。方法选择T2DM患者46例(T2DM组),其中伴肥胖者23例(T2DM+FAT组)、单纯T2DM 23例(单纯T2DM组);非T2DM患者56例(非T2DM组),其中单纯非T2DM患者33例(单纯非T2DM组)、伴肥胖者23例(非T2DM+FAT组)。采用RT-PCR技术检测各组腹部皮下及大网膜脂肪组织的HO-1 mRNA表达,蛋白免疫印迹技术检测其HO-1蛋白表达,分析其与临床及实验室相关指标的关系。结果①在腹部皮下及网膜脂肪组织中,单纯T2DM组及T2DM+FAT组的HO-1 mRNA表达量分别低于单纯非T2DM组及非T2DM组+FAT组,且T2DM+FAT组低于单纯T2DM组(P均<0.05)。网膜脂肪组织中的HO-1 mRNA表达量与BMI、HbA1c呈负相关(P均<0.01)。②在腹部皮下和网膜脂肪组织中,单纯T2DM组及T2DM+FAT组的HO-1蛋白表达量分别低于单纯非T2DM组及非T2DM组+FAT组(P均<0.05),其中T2DM+FAT组网膜脂肪组织的表达量与BMI、FPG、TG呈负相关(P均<0.05)。③相关分析显示,T2DM患者网膜脂肪组织HO-1蛋白表达量与血清脂联素水平呈正相关(P<0.05)。结论 T2DM患者的HO-1基因及蛋白表达明显降低,BMI越高,其表达量越低,HO-1表达降低可能与胰岛素抵抗及T2DM发生、发展有关。 Objective To observe the heme oxygenase-1 （HO-1） mRNA and protein expression in subcutaneous and omental adipose tissue of type 2 diabetic patients and their relation with serum adiponectin level, and other clinical indexes. Methods 23 type 2 diabetic patients with obesity, 23 type 2 diabetic patients, 33 controls with obesity and 23 controls were selected . The HO-1 mRNA expression level in adipose tissue was assayed by RT-PCR, and HO-1 protein expression level was assayed by Western blot. Being usedlinear correlation analysis, their correlations were observed. Results ①The expressions of HO-lmRNA in subcutaneous adipose tissue of type 2 diabetic patients with obesity were significantly lower （all P 〈0.05）, and the level in omental adipose tissue was negatively correlated to BMI （P 〈0. 01 ）. ② Type 2 diabetic patients and those with obesity showed significantly lower expressions of HO-1 protein in subcutaneous and omental adipose tissue comparing with control groups （ all P 〈 0.05 ）, and the level in omental adipose tissue of type 2 diabetic patients with obesity were negatively correlated to BMI, FPG, TG （ all P 〈 0.05 ）.③The expression level of HO-1 protein in omental adipose tissue of type 2 diabetic patients was positively correlated to the level of serum adiponectin （ P 〈 0.05 ）. Conclusions The levels of HO-lmRNA and protein in type 2 diabetic patients are significantly lower than those of controls. The higher level of BMI is, the lower of HO-1 expression is. The low expression of HO-1 may be correlated with the occurrence and development of insulin resistance and DM.
作者 李倩倩 李慧敏 钟继娟 缪珩
机构地区 南京医科大学第二附属医院
出处 《山东医药》 CAS 2012年第45期7-10,共4页 Shandong Medical Journal
基金 江苏省教育厅自然科学研究面上项目(11KJB320003)
关键词 糖尿病 2型 血红素加氧酶-1 胰岛素抵抗 脂联素 diabetes mellitus, type 2 hemeoxygenase-1 insulin resistance adiponeetin
分类号 R587.1 [医药卫生—内分泌]
  • 相关文献

参考文献8

  • 1Kaneto H, Kawamori D, Nakatani Y, et al. Oxidative stress and the JNK pathway as a potential therapeutic target for diabetes[ J]. Drug News Perspect, 2004,17 (7) :447-453.
  • 2Nicolai A, Li M, Kim DH, et al. Heme oxygenase-1 induction remodels adipose tissue and improves insulin sensitivity in obesity-induced diabetic rats[J]. Hypertension, 2009,53 (3) :508-515.
  • 3Li M, Kim DH, Tsenovoy PL, et al. Treatment of obese diabetic mice with a heme oxygenase inducer reduces visceral and subcutaneous adiposity, increases adiponectin levels, and improves insulin sensitivity and glucose tolerance [ J ]. Diabetes, 2008,57 (6) : 1526- 1535.
  • 4李倩倩,蒋秀琴,李慧敏,缪珩.2型糖尿病患者皮下、大网膜脂肪组织脂联素mRNA表达变化及意义[J].南京医科大学学报（自然科学版）,2011,31(3):404-409. 被引量：3
  • 5Gauthier MS, O'Brien EL, Bigornia S, et al. Decreased AMP-activated protein kinase activity is associated with increased inflammation in visceral adipose tissue and with whole-body insulin resistance in morbidly obese humans [ J ]. Biochem Biophys Res Commun, 2011,404( 1 ) :382-387.
  • 6Murano I, Barbatelli G, Parisani V, et al. Dead adipocytes, detected as crown-like structures, are prevalent in visceral fat depots of genetically obese mice[J]. J Lipid Res, 2008,49(7) :1562-1568.
  • 7Kruger AL, Peterson S J, Schwartzman ML, et al. Up-regulation of heme oxygenase provides vascular protection in an animal model of diabetes through its antioxidant and antiapoptotic effects[J]. J Pharmacol Exp Ther, 2006,319(3) :1144-1152.
  • 8Bruce CR, Carey AL, Hawley JA, et al. Intramuscular heat shock protein 72 and heme oxygenase-1 mRNA are reduced in patients with type2 diabetes: evidence that insulin resistance is associated with a disturbed antioxidant defense mechanism [ J ]. Diabetes, 2003,52 (9) :2338-2345.

二级参考文献18

  • 1戈敏娟,高小亚,马向华.单纯性肥胖及2型糖尿病患者脂联素水平的研究[J].南京医科大学学报（自然科学版）,2005,25(6):406-408. 被引量：8
  • 2Bastard JP,Maachi M,Lagathu C, et al. Recent advances in the relationship between obesity,inflammation, and insulin resistance [J]. Eur Cytokine Netw, 2006,17(1) :4-12.
  • 3Yamauchi T, Kamon J, Waki H, et al. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity [J]. Nat Med, 2001,7(8) :941-946.
  • 4Maeda N, Shimomura I, Kishida K,et al. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30 [J]. Nat Med, 2002,8(7) :731-737.
  • 5Hotta K, Funahashi T, Arita Y, et al. Plasma concentrations of a novel,adipose-specific protein, adiponectin, in type 2 diabetic patients[J]. Arterioscler Thromb Vasc Biol,2000, 20(6) : 1595-1599.
  • 6Tanko LB, Bruun JM, Alexandersen P, et al. Novel associations between bioavailable estradiol and adipokines in elderly women with different phenotypes of obesity: implications for atherogenesis [ J ]. Circulation, 2004,110 (15) :2246-2252.
  • 7Maeda K, Okubo K, Matsuzawa Y, et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (adipose most abundant gene transcript 1)[J] .Biochem Biophys Res Commun, 1996,221 (2) : 286-289.
  • 8Kissebah AH, Sonnenberg GE, Myklebust J, et al.Quantitativetrait loci chromosome 3 and 17 influence pheno- types of the metabolic syndrome [J]. Proc Natl Acad Sci USA, 2000,97(26) : 14478-14483.
  • 9Comuzzie AG, Funahashi T, Sonnenberg G, et aI. The genetic basis of plasma variation in adiponectin, a global endophenotype for obsety and the metabolic syndrome [J]. J Clin Endocrinol Metab,2001,86(9):4321-4325.
  • 10Lihn AS, Bruun JM, He G, et al. Lower expression of adiponectin mRNA in visceral adipose tissue in lean and obese subjects [ J ]. Mol C ell Endoerinol, 2004,219 ( 1-2): 9-15.

共引文献2

同被引文献21

  • 1Teede H,Deeks A,Moran L. Polyeystic ovary syndrome:a complex condition with psychological, reproductive and metabolic manifesta- tions that impacts on health across the lifespan[J]. BMC Med, 2010, 8(1) :41-50.
  • 2Kelly C C,Lyall H,Petrie J R,et al. Low grade chronic in- flammation in women with polycystic ovarian syndrome[J]. J Clin Endocrinol Metab,2001,86(6) :2453-2455.
  • 3Grochot-Przeczek A, Dulak J,Jozkowicz A. Haem oxygenase- 1 : non-canonical roles in physiology and pathology[J]. Clin Sci (Lond), 2012,122 (3) : 93-103.
  • 4Lorenzo C, Hazuda H P, Haffner S M. Insulin resistance and excess risk of diabetes in Mexican-Americans: the San An- tonio Heart Study[J]. J Clin Endocrinol Metab, 2012,97 (3) : 793-799.
  • 5Moran L J,Misso M L,Wild R A,et al. Impaired glucose tol- erance,type 2 diabetes and metabolic syndrome in polycystic ovary syndrome: a systematic review and meta-analysis[J]. Hum Reprod Update,2010,16(4) :347-363.
  • 6Gao H,Meng J,Xu M,et al. Serum heat shock protein 70 con- centration in relation to polycystic ovary syndrome in a non- obese chinese population[J]. PLoS One,2013,8(6) :e67727.
  • 7Murri M, Luque-Ramirez M, Insenser M, et al. Circulating markers of oxidative stress and polycystic ovary syndrome(P- COS) :a systematic review and meta-analysis[J]. Hum Reprod Update, 2013,19(3) : 268-288.
  • 8Nicolai A, Li M, Kim D H, et al. Heine oxygenase-1 induction remodels adipose tissue and improves insulin sensitivity in o- besity-induced diabetic rats[J]. Hypertension, 2009,53 (3) : 508-515.
  • 9Gonzalez F, Rote N S, Minium J, et al. Reactive oxygen spe- cies-induced oxidative stress in the development of insulin re- sistance and hyperandrogenism in polycystic ovary syndrome [J]. J Clin Endoerinol Metab, 2006,91 (1) : 336-340.
  • 10Inoguchi T,Li P,Umeda F, et al. High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C-dependent activation of NAD(P)H oxidase in cultured vascular cells[J]. Diabetes, 2000,49 (11): 1939-1945.

引证文献2

二级引证文献22

山东医药
2012年 第45期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部