摘要
从曼地亚红豆杉(Taxus x media)细胞中提取总RNA,通过RT-PCR克隆了dbtnbt基因,测序结果表明dbtnbt基因全长为1 317 bp;将dbtnbt基因与原核表达载体pET32a(+)连接,成功构建了重组质粒pET32a(+)-dbtnbt,并将其转入E.coli BL21 plysS中进行表达,dbtnbt基因的表达产物分子量约为48.4 ku,与预测大小一致。
The total RNA of Taxus x media cells were isolated and used to synthesize dbtnbt by RT-PCR.The total lenght of dbtnbt was 1 317 bp.Then the fragments of dbtnbt was ligated into pET32a（＋） to produce the recombinant plasmid pET32a（＋）-dbtnbt,which was transformed into E.coli BL21 plysS for induction expression.The SDS-PAGE results showed that the recombinant plasmid pET32a（＋）-dbtnbt was successfully constructed and expressed;and the molecular weight of the expression product of dbtnbt was about 48.4 ku,which was corresponding to the deduced size.
出处
《湖北农业科学》
北大核心
2012年第9期1912-1915,共4页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(NSFC 20776058
20906036)
中国博士后科学基金项目(20100471183)
