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上海市猪圆环病毒2型全基因组的克隆与序列分析 被引量：2

CLONING AND SEQUENCE ANALYSIS OF THE COMPLETE GENOMES OF 12 SHANGHAI STRAINS OF PORCINE CIRCOVIRUS TYPE 2
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摘要 参照国外发表的猪圆环病毒2型(Porcine circovirus type 2,PCV-2)全基因组序列,设计合成了1对特异性引物,从上海市临床病料中提取PCV-2基因组DNA,进行PCR全基因扩增。回收PCR产物,将其插入pMD18-T载体,并对筛选出的阳性质粒进行测序。应用DNAStar序列分析软件,对所测PCV-2序列与GenBank中登录的国内外PCV-2毒株进行同源性比较。结果显示,PCV-2上海株与国内外毒株的核苷酸同源性高达95.0%～100%。进化树分析结果显示,其中9株PCV-2病毒属于PCV-2b亚型,3株PCV-2病毒属于PCV-2a亚型,且9株PCV-2b亚型毒株部分核苷酸位点显示其属于强毒力毒株。研究表明,上海市PCV-2感染以PCV-2b亚型为主,且氨基酸位点表明其具有较高的毒力。 According to the published genome sequences of PCV-2, a pair of specific primers was designed to amplify the full length genome from clinical pig tissues collected from Shanghai area. The obtained fragments were cloned into pMD18-T vector for sequence analysis. These 12 PCV-2 Shanghai strains shared 95.0%- 100% nucleotide identities with other PCV-2 strains inGenBank. Phylogenetic analysis showed that 9 Shanghai strains belonged to PCV-2b subtype and 3 strains to PCV-2a subtype. Variations at some amino acid positions suggested high virulence of 9 PCV-2b strains.
作者 刘健 鞠厚斌 张维谊 杨德全 王建 刘佩红 周锦萍
机构地区 上海市动物疫病预防控制中心
出处 《中国动物传染病学报》 CAS 2012年第3期73-77,共5页 Chinese Journal of Animal Infectious Diseases
关键词 猪圆环病毒2型 基因组 序列分析 Porcine circovirus type 2 （PCV-2） genome sequence analysis
分类号 S852.659.2 [农业科学—基础兽医学] Q78 [生物学—分子生物学]
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参考文献10

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共引文献33

同被引文献21

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中国动物传染病学报
2012年 第3期

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