C-Met蛋白在结直肠癌中的表达及其与肿瘤血管生成的关系

Correlation between c-Met expression and tumor angiogenesis in colorectal cancer

目的:检测结直肠癌组织中c-Met蛋白表达情况并计数其微血管密度(microvessel density,MVD)值,探讨两者之间的相互关系,评价其与结直肠癌临床病理特征的关系.方法:对我院病理科2009-2010年经手术切除经病理证实的结直肠癌、癌旁组织及正常组织标本各40例,采用Western blot和SP法分别检测3种组织中c-Met蛋白表达和CD34标记的MVD.结果:c-Met蛋白在结直肠癌组织中阳性表达率明显高于结直肠癌旁组织与正常黏膜组织(χ2=29.39,P≤0.01).结直肠癌组织中MVD明显高于结直肠癌旁组织和正常黏膜组织(H=90.468,P≤0.01).结直肠癌组织c-Met蛋白表达和MVD与浸润深度(P<0.05)、Dukes分期(P<0.05)、淋巴结转移及远处转移(P<0.05)密切相关,c-Met阳性表达的结直肠癌组织中MVD显著高于c-Met阴性者(t=3.126,P<0.01).结论:结直肠癌患者的c-Met及MVD值高于癌旁组织及正常黏膜组织,且随着结直肠癌的不断进展,两者在结直肠癌的侵袭和转移中发挥着重要作用,联合检测c-Met的表达及MVD可能有助于结直肠癌临床评价和预后判断.

AIM： To investigate the correlation between ex- pression of c-Met protein and microvessel den- sity （MVD） in colorectal carcinoma and to assess their clinical significance. METHODS： Immunohistochemical SP method was used to detect the expression of c-Met pro- tein and CD34 （for assessment of MVD） in 40 cases of colorectal carcinoma, 40 cases of tumor- adjacent neoplastic tissue and 40 cases of normal colorectal tissue.RESULTS： The positive rate of c-Met expres- sion in colorectal carcinoma was significantly higher than that in adenoma and normal tissue （3（2 = 29.39, P 〈 0.01）. The MVD in colorectal car- cinoma was also significantly higher than that in adenoma and normal tissue （H = 90.468, P 〈 0.01）. C-Met expression and MVD had no correlation with tumor site and histological stage, but were closely related to Dukes stage, depth of invasion, lymph node metastasis and distant metastasis （all P 〈 0.05）. The MVD in c-Met-positive rectal can- cer was higher than that in c-Met-negative rectal cancer （t ^- 3.126, P 〈 0.01）. CONCLUSION： C-Met possibly plays an im- portant role in the invasion and metastasis of colorectal carcinoma. Combined detection of c-Met expression and MVD may be helpful for judging prognosis in colorectal carcinoma.