摘要
目的:探讨茯苓多糖对小鼠外周血免疫球蛋白IgA、IgG和IgM合成水平的影响。方法:50只成年昆明种雄性小鼠随机分成5组,生理盐水对照组与盐酸左旋咪唑对照组分别灌注生理盐水与2.5g/L盐酸左旋咪唑溶液,茯苓多糖低、中、高剂量组分别灌注1g/L、2g/L、4g/L的茯苓多糖溶液,持续一周后摘眼球取血,采用ELISA法测定各组小鼠外周血清中IgA、IgG和IgM含量。结果:茯苓多糖处理组IgA、IgG和IgM水平高于生理盐水对照组,组间差异均有统计学意义(P<0.01);IgA、IgG和IgM水平与茯苓多糖剂量呈正相关(IgA:rs=0.934,P<0.01;IgG:rs=0.943,P<0.01;IgM:rs=0.939,P<0.01)。结论:茯苓多糖可促进小鼠血清IgA、IgG和IgM的生物合成,且存在剂量-效应关系,作用随茯苓多糖浓度的增大而增强。
Objective:To explore the influence of Pachyman on biosynthesis level of mice peripheral blood im- munoglobulins IgA, IgG and IgM. Methods: 50 adult male Kunming mice were randomly divided into five groups, in which the saline and levamisole hydrochloride control group were respectively perfused with saline and 2.5g / L levamisole hydrochloride solution, pachyman in low, medium and high dose groups, were re- spectively perfused with Pachymaran of lg /L, 2g / L and 4g / L. A week later, the blood of the eyeball was taken out to measure the concentration of mice peripheral blood immunoglobulins IgA, IgG and IgM with the ELISA method. Results: Pachyman processing groups IgA, IgG and IgM levels were higher than the saline control group, and group differences were statistically significant (P 〈0. 01); IgA, IgG and IgM levels Pachymaran dose was positively correlated(IgA: rs : 0. 934, P〈0.01; IgG: rs = 0. 943, P d0.01; IgM: rs : 0. 939, P d0. 01). Conclusion: Pachyman can promote the biosynthesis of mice serum IgA, IgG and IgM, and the dose - response relationship is obvious, The effect is enhanced with the increase of the Pchyman concentration.
基金
2010年湖南省大学生研究性学习和创新性实验计划项目(2010-515)