酶解法提取玉米须中总黄酮工艺的优化

Optimization of Enzymatic Hydrolysis Extraction Method of Total Flavonoids from Corn Stigma

以乙醇溶液为提取剂,采用纤维素酶酶解的方法从玉米(Zea mays)须中提取总黄酮。设计单因素试验分析了酶用量、酶解温度、酶解时间、pH、乙醇体积分数和料液比等6个因素对玉米须中总黄酮提取率的影响,采用响应面法进一步优化总黄酮提取的工艺条件。结果表明,酶法提取玉米须中总黄酮的最佳工艺条件为每5.0 g干燥的玉米须粉末中加入3.0 g纤维素酶、酶解温度45.00℃、酶解时间149.1 min、pH4.49、体积分数30%的乙醇溶液作为提取溶剂、料液比1∶20(m∶V,g/mL),此条件下玉米须总黄酮的提取率可达0.837%。

Using ethanol solution as extractant,total flavonoids were extracted from corn（Zea mays） stigma by cellulose digestion.Effects of enzyme dose,digesting temperature,digesting time,pH,volume fraction of ethanol and solid to liquid ratio on the yield of total flavonoids were studied by single factor tests and response surface method.The optimized extraction conditions were as follows,digesting temperature,45.00 ℃;digesting time,149.1 min;pH,4.49;volume fraction of ethanol,30%;solid to material ratio,1∶20（m∶V,g/L）.Under these conditions,the yield of total flavonoids was 0.837%.