外植体类型和植物生长调节剂浓度对红金银花愈伤组织诱导的影响

Effects of Explants Type and Plant Growth Regulator's Concentration on Callus Induction of Lonicera japonica var.chinensis

以红金银花(Lonicera japonica var.chinensis)顶芽、当年生幼茎和叶片为试验材料,利用仿自然气候的培养条件,采用L9(34)正交试验设计,考察了外植体类型和培养基中添加植物生长调节剂的浓度对愈伤组织诱导的影响。结果表明,外植体类型对红金银花组织培养的污染率及愈伤组织的诱导率均有影响,以顶芽为外植体时污染率低,愈伤组织诱导率高,为最佳外植体。优化后的愈伤组织诱导培养基中KT、6-BA、α-NAA浓度分别为1.5、1.5、0.5 mg/L。

Using the terminal bud,young stem and leaf of Lonicera japonica var.chinensis as experimental material,the effects of explants type and plant growth regulator＇s concentration in culture medium on callus induction were studied under the culture condition of imitating nature climate through L9（34） orthogonal design.The main results showed that explants type affect both pollution rate and callus inducing rate.The terminal bud was the best explant as it was with low pollution rate and high inducing rate.The optimized concentration of KT,6-BA and α-NAA in callus induction medium was 1.5,1.5,0.5 mg/L,respectively.