摘要
为研究马鞭草(Verbena officinalis L.)提取液对大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)的抑制作用,将菌悬液与不同浓度马鞭草提取液混合均匀涂布到细菌培养基上进行生物学测定。结果表明,马鞭草提取液能抑制2种细菌,对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度(MIC)分别为500.0 mg/mL和250.0 mg/mL;药液浓度分别不低于500.0 mg/mL和250.0 mg/mL时,马鞭草提取液对大肠杆菌和金黄色葡萄球菌的抑菌率均为100.00%。
The bacteria liquid with 103CFU / mL was prepared by flat calculating colony,the mixture of bacteria liquid and Verbena officinalis L,with different concentrations were smeared on normal bacteria culture medium,the influence of V.officinalis on the growth of Escherichia coli and Staphylococcus aureus was researched.The results showed that growth of E.coli and S.aureus was significantly inhibited by V.officinalis,the MIC of V.officinalis to E.coli and S.aureus was 500.0 mg / mL and 250.0 mg / mL separately;the inhibitory rate was 100.00% under MIC.
出处
《湖北农业科学》
北大核心
2012年第20期4524-4526,共3页
Hubei Agricultural Sciences
基金
河南省教育厅自然科学基金项目(2010B180034)
关键词
马鞭草提取液
大肠杆菌
金黄色葡萄球菌
抑菌率
最小抑菌浓度(MIC)
Verbena officinalis L.
Escherichia coli
Staphylococcus aureus
inhibitory rate
minimal inhibitory concentration(MIC)