槲皮素对烟雾吸入性肺损伤治疗作用的实验研究

Therapeutic effects of quercetin against smoke inhalation injury in rats

目的探讨槲皮素在烟雾吸入性损伤中对肺的保护作用。方法雄性SD大鼠42只采用随机数字表法随机分为3组:A组(6只)、B组(18只)、C组(18只),后两组均再按照致伤后6h、24h和48h三个时间段各分为三个亚组(B1、B2、B3、C1、C2、C3),每亚组6只大鼠。C组在吸入性损伤处理后3h腹腔注射槲皮素(200mg/kg),B组于致伤后3h腹腔注射等量的0.9%氯化钠溶液,A组无处理。A组、B1组、C1组于致伤后6h,B2组、C2组于伤后24h,B3组、C3组于伤后48h留取大鼠股动脉血液样本5mL离心取血清检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)含量,留取肺组织制备肺组织匀浆后测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)和肺髓过氧化物酶(MPO)活性。取部分肺组织经4%甲醛溶液固定后做病理苏木精-伊红染色切片光镜观察。结果 C组各个时段TNF-α、IL-6、MDA、MPO水平较B组相应时段降低,差异具有统计学意义(P﹤0.01),SOD及IL-10水平增高,差异具有统计学意义(P﹤0.01)。光镜下B组各时段肺泡间隔增宽,炎细胞浸润,局灶性出血,C组各时段较B组相应时段炎细胞浸润均有减轻。结论槲皮素可能通过减少炎症介质及自由基的产生对烟雾吸入性肺损伤起到保护作用,且随着时间的增加效果更明显。

Objective To study the therapeutic effects of quercetin against smoke inhalation injury in rats. Methods Forty-two male SD rats were randomly divided into three groups： group A（n=6）, group B（n=18）, and group C（n=18）. The latter two groups in accordance with 6 h, 24 h and 48 h after injury were each divided into three groups （B1, B2, B3, C1, C2, C3） （n=6）. 3 h after smoke inhaled injured, the rats in group C were all injected intraperitoneally with quercetin（200 mg/kg）, and the group B were injected the same volume normal saline. The control group received no treatment. At 6 h after injured in group A、 B1 and C1, 24 h after injured in group B2 and C2, and 48 h after injured in group B3 and C3, Collected the blood samples 5 mL form femoral artery and lung tissue in rats. The levels of tumor necrosis factor-α（TNF-α）, interleukin-6 （IL-6）and interleukin-10（IL-10） in serum and malondialdehyde （MDA）,myeloperoxidase （MPO） and Superoxide dismutase（SOD） in lung tissue were determined. The lung tissues were examined under microscope. Results The levels of TNF-α、IL-6 and MDA at every phase in group C were lower than those in group B （P〈0.01）,and the SOD and IL-10 were higher （P〈0.01）. Pathology showed thickened alveolar space, inflammatory cells effusion, and local hemorrhage in group B, Inflammatory granulocytes were improved in group C. Conclusion Quercetin has a protective effect on smoke inhalation injured by scavenging free radical and inhibiting proinflammatory cytokines.