吉林白鹅α干扰素基因的克隆与序列分析

Cloning and Sequence Analysis of Interferon-α Gene in Jilin White Goose

从吉林白鹅(Anser cygnoides)肝脏组织提取基因组DNA,应用Primer 5.0软件设计一对加入EcoRⅠ和BamHⅠ酶切位点的特异性引物,PCR扩增得到α干扰素基因(IFN-α),将PCR产物克隆至pMD18-T克隆载体,转化DH5α感受态细胞,挑取阳性菌落进行鉴定和测序。结果表明,IFN-α已被成功克隆。序列分析结果显示该基因序列与GenBank中已知的狮头鹅(A.anser)IFN-α序列(登录号HQ009755)同源性为99.5%。

The interferon-α gene（IFN-α）fragment was amplified with a pair of specific primers within BamHⅠ and EcoRⅠ restriction sites by PCR with the genome DNA extracted from liver of Jilin white goose（Anser cygnoides）.PCR product was cloned into vector pMD18-T and used to transform DH5α competent cell.Sequential analysis showed that the IFN-α was successfully cloned.Its homogeneity with reported A.anser IFN-α gene in GenBank（Accession No.HQ009755） was 99.5%.