柽柳ThPR1基因的克隆与表达分析

Clone and expression analysis of ThPR1 gene in Tamarix hispida

通过对盐胁迫后刚毛柽柳(Tamarix hispida)7个转录组分析,鉴定获得病程蛋白相关基因PR1(命名为ThPR1)全长cDNA序列,该基因全长756 bp,编码区长537 bp,编码含178个氨基酸的蛋白质,分子质量为20.53 ku,理论等电点(pI)为9.75。为了研究该基因对逆境胁迫的应答情况,采用实时定量RT-PCR技术分析了刚毛柽柳在PEG、NaCl胁迫及外源ABA处理后不同组织中基因的表达。结果表明,NaCl会诱导ThPR1基因在根和叶中的表达,而PEG和ABA处理则抑制该基因在根和叶中的表达。

Pathogenesis-related proteins（PRs） in plants are proteins in plants that induced by biological or abiotic stresses.In this study,the full length cDNA of a PR1（named ThPR1） gene was isolated from seven transcriptome cDNA librarys of T.hispida under NaHCO3 stress.The ThPR1 cDNA was 756 bp,and open reading frame（ORF） was 537 bp,encoding a protein of 178 amino acid residues with the molecular mass of 20.53 ku and theoretical pI of 9.75.The expressions of ThPR1 in T.hispida responding to NaCl,PEG and ABA were further investigated using quantitative RT-PCR.The results showed that ThPR1 was induced in roots and leaves of T.hispida under NaCl stress,but inhibited under PEG and ABA treatments.