期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

腺苷A3受体激动剂对新生期母婴分离肠易激综合征大鼠内脏高敏感的作用

下载PDF
导出
摘要 目的研究腺苷A3受体在新生期母婴分离肠易激综合征(IBS)大鼠模型内脏高敏感中的作用。方法采用新生期Sprague-Dawley雄性大鼠和母婴分离方法建立内脏高敏感模型,分别用腺苷A3受体激动剂2-Cl-IB-MECA及DMSO溶剂作用后,用行为学观察和腹部回缩反射评分来检测内脏痛反应,用免疫组化方法检测腰骶段脊髓背角神经元c-fos蛋白的表达数量和远端结肠中5-羟色胺阳性细胞数目,用ELISA方法检测远端结肠中5-羟色胺的含量。结肠行常规组织学检测。结果与DMSO组比较,2-Cl-IB-MECA组大鼠在40、60和80 mmHg结直肠压力扩张下的AWR评分显著减低(P<0.05),脊髓背角c-fos蛋白免疫反应阳性神经元数目显著减少(P<0.05),结肠黏膜5-羟色胺阳性细胞数目和5-羟色胺含量均显著减少(P<0.05)。两组大鼠在结直肠压力扩张前后,结肠均未见组织损伤或炎症。结论腺苷A3受体激活可能通过减少结肠5-羟色胺的作用未减轻IBS大鼠模型的内脏高敏感,腺苷A3受体可能是治疗IBS内脏高敏感的新靶点。
作者 杨名诗 任天华
机构地区 广东医学院附属医院消化内科
出处 《广东医学》 CAS CSCD 北大核心 2013年第6期844-847,共4页 Guangdong Medical Journal
关键词 肠易激综合征 内脏高敏感 腺苷A3受体 5-羟色胺
分类号 R574.4 [医药卫生—消化系统]
  • 相关文献

参考文献18

  • 1CREMON C, CARINI G, WANG B, et al. Intestinal serotonin re- lease, sensory neuron activation, and abdominal pain in irritable bowel syndrome [ J ]. Am J Gastroenterol, 2011, 106 (7) : 1290 - 1298.
  • 2CHRISTOFI F L, K1M M, WUNDERLICH J E, et al. Endoge- nous adenosine differentially modulates 5 - hydroxytryptamine re- lease from a human enterochromaffin cell model [ J ]. Gastroenter- ology, 2004, 127(1) : 188 -202.
  • 3REN T H, WU J, YEW D, et al. Effects of neonatal maternal separation on neurochemical and sensory response to colonic dis- tension in a rat model of irritable bowel syndrome[ J]. Am J Physi- ol Gastrointest Liver Physiol, 2007, 292 (3) : G849 - G856.
  • 4ZHOU Q, VERNE G N. New insights into visceral hypersensitivi- ty-- clinical implications in IBS [ J ]. Nat Rev Gastroenterol Hepatol, 2011, 8(6 ) : 349 -355.
  • 5CAMILLERI M. Serotonin in the gastrointestinal tract [ J]. Curr Opin Endocrinal Diabetes Obes, 2009, 16( 1 ) : 53 -59.
  • 6王静,诸琦.5-羟色胺受体及转运体在肠易激综合征发病机制中的作用[J].上海交通大学学报（医学版）,2008,28(8):1051-1054. 被引量：15
  • 7SPILLER R C. Targeting the 5 -HT(3) receptor in the treatment of irritable bowel syndrome[ J]. Curt Opin Pharmacol, 2011, 11 (1) : 68 -74.
  • 8AL JUDAIBI B, CHANDE N, GREGOR J. Safety and efficacy of te- gaserod therapy in patients with irritable bowel syndrome or chronic constipation[J]. Can J Clin Pharmacol, 2010, 17(1) : e194 -e200.
  • 9ELSENBRUCH S. Abdominal pain in irritable bowel syndrome: a review of putative psychological, neural and neuro - immune mechanisms [ J ]. Brain Behav Immun, 2011,25 ( 3 ) : 386 - 394.
  • 10BLACKBURN M R, VANCE C O, MORSCHL E, et al. Adeno- sine receptors and inflammation[ J]. Handb Exp Pharmacol, 2009 (193) : 215 -269.

二级参考文献22

  • 1Coates MD, Mahoney CR, Linden DR, et al. Molecular defects in mucosal serotonin content and decreased serotonin reuptake transporter in ulcerative colitis and irritable bowel syndrome [ J]. Gastroenterology, 2004, 126 (7): 1657-1664.
  • 2Mawe G, Coates M, Moses P. Intestinal serotonin signalling in irritable bowel syndrome [ J ]. Aliment Pharmacol Ther, 2006, 23 (8) : 1067 -1076.
  • 3Chey WD, Chey WY, Heath A, et al. Long-term safety and efficacy of alosetron in women with severe diarrhea-predominant irritable bowel syndrome[J]. Am J Gastroenterol, 2004, 99 (11): 2195- 220.q
  • 4Baker DE. Rationale for using serotonergic agents to treat irritable bowel syndrome[ J]. Am J Health Syst Pharm ,2005,62 (7) : 700 - 711.
  • 5Greenwood V, Meerveld B, Venkova K, et al. Activation of peripheral 5-HT receptors attenuates colonic sensitivity to intraluminal distension[ J]. Neurogastroenterol Motil, 2006, 18 ( 1 ): 76 - 86.
  • 6MeLaughlin J, Houghton L. The rationale, efficacy and safety evidence for tegaserod in the treatment of irritable bowel syndrome [ J ]. Expert Opin Drug Saf, 2006, 5 (2) : 313 -327.
  • 7Thompson CA. Novartis suspends tegaserod sales at FDA's request [J]. Am J Health Syst Pharm, 2007, 64 (10) :1020.
  • 8Pan H, Gershon MD. Activation of intrinsic afferent pathways insubmucosal ganglia of the guinea pig small intestine [ J ]. J Neurosci, 2000, 20 (9) : 3295 -3309.
  • 9Tonini M, Vicini R, Cervio E, et al. 5-HT7 Receptors modulate peristalsis and accommodation in the guinea pig ileum[ J ]. Gastroenterology, 2005, 129 (5): 1557-1566.
  • 10Mahe C, Loetscher E, Dev KK, et al. Serotonin 5-HT7 receptors coupled to induction of interleukin-6 in human microglial MC-3cells [ J ]. Neuropharmacology, 2005, 49 ( 1 ) : 40 - 47.

共引文献14

广东医学
2013年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部