辛伐他汀与Bio-Oss骨粉混合物对种植体周围缺损骨重建中破骨细胞的作用

Effects of simvastatin and Bio-Oss composite on osteoclasts in implant neighboring defect region during bone remodeling

目的探讨辛伐他汀与Bio-Oss骨粉复合物对兔胫骨种植体周围骨缺损内骨重建中破骨细胞的作用。方法选择24只健康日本大耳白兔,分别在双侧胫骨体部上1/3处各植入1枚种植体,并在种植体一侧建立骨缺损区。分为S0.5(0.5mg辛伐他汀+Bio-Oss骨粉),S1.0(1.0mg辛伐他汀+Bio-Oss骨粉),CB(单纯Bio-Oss骨粉),C0(空白对照)组,每组6只。分别于术后4、8周各处死动物3只,行组织学观察和破骨细胞特殊染色计数分析。结果组织学观察:各组8周均比4周新生骨面积大。4、8周时S1.0、S0.5较CB、C0新生骨面积大。抗酒石酸酸性磷酸酶(TRAP)特殊染色:各组4周破骨细胞计数差异具有统计学意义,S1.0组破骨细胞计数最多;C0组最少。结论辛伐他汀与Bio-Oss骨粉复合物可促进骨重建,且促进骨缺损中破骨细胞的生成。

Objective To explore the effects of simvastatin and Bio-Oss composite on the osteoclasts （OCs） in tibial implant neighboring defect region during bone remodeling in rabbits. Methods Twenty-four healthy Japanese white rabbits were subjected to placement of implants in the upper third region of bilateral tibia for establishment of neighboring bone defect regions. These rabbits were randomly assigned to receive 0.5 mg simvastatin and Bio-Oss composite （group S0.5, n=6）, 1.0 mg simvastatin and Bio-Oss composite （group S10, n=6） and Bio-Oss injection alone （group CB, n=6） and nil treatment （group Co, n=6）, respectively. Three rabbits of each group were sacrificed at weeks 4 and 8 for histological examination and osteoclast count by tartrate-resistant phosphatase （TRAP） staining. Results Histological examination evidenced that the area with osteogenesis increased at week 8 and that groups S05 and S10 yielded a marked osteogenesis area compared with groups CB and Co at weeks 4 and 8. TRAP staining suggested a sta- tistically significant difference in osteoclast count among all groups at week 4. Groups S1.0 and Co were characterized by the highest and lowest osteoclasts count, respectively. Conclusion The simvastatin and Bio-Oss composite may promote bone remodeling and stimulate osteoclast growth in the bone defect region.