重组人生长激素对人脑颅咽管瘤细胞GHR基因表达的调控

Effects of rhGH on GHR expression in human craniopharyngioma cells

目的探讨重组人生长激素对人脑颅咽管瘤细胞GHR基因表达的影响。方法分为实验组(rh-GH处理)与对照组(rhGH未处理),采用RT-PCR检测颅咽管瘤细胞GHR mRNA水平变化,Western blot检测GHR蛋白在颅咽管瘤细胞中的表达变化。结果 rhGH处理后的颅咽管瘤细胞表达的GHR蛋白较对照组增加(P<0.05),RT-PCR结果显示,实验组颅咽管瘤细胞GHR mRNA表达较对照组增加(P<0.05)。结论 rhGH可使颅咽管瘤细胞GHR表达增加,这可能为颅咽管瘤患者进行rhGH替代治疗提供一个临床指导依据。

Objective To investigate the role of recombinant human growth hormone （rhGH） in the regulation of GHR expression in craniopharyngioma cells in human. Methods Craniopharyngioma cells were divided into experimental group and control group, in which they were treated with and without rhGH, respectively. The expression of GHR was as- sessed by Western blot and RT - PCR. Results There were significantly up - regulation of GHR in both protein and mR- NA in experimental group according to Western blot and RT - PCR （P 〈 0. 05）. Conclusion In human craniopharyngio- ma cells, rhGH dramatically increases GHR expression, which may provide evidences for rhGH replacement therapy for craniopharyngioma.