青蒿有效部位的提取工艺优化及驱蚊效果评价

Optimization of Extraction Technology of Effective Parts in Artemisia annua and Evaluation of Its Repellent Effect

目的:优选青蒿有效部位的提取工艺并评价其驱蚊效果。方法:采用单因素试验筛选青蒿驱蚊有效部位的提取方法;以挥发油得率为指标,通过正交试验考察时间、温度及压力对提取工艺的影响,确定最佳提取工艺。以有效保护率为指标,评价青蒿提取物的驱蚊效果。结果:采用超临界CO2萃取工艺,其最佳萃取工艺为温度60℃,压力30 MPa,时间2 h,挥发油得率约0.35%。制备的青蒿驱蚊提取物(质量浓度>0.05 g·mL-1)在2 h内有效保护率达99%。结论:优选的提取工艺稳定合理,具有较好的驱蚊作用。

Objective：To optimize extraction technology of effective parts in Artemisia annua and evaluate its repellent effect.Method：Extraction method of repellent effective parts in A.annua was screened by single factor test;With yield of volatile oil as index,effects of time,temperature and pressure on extraction technology was investigated by orthogonal test.Repellent effect of the extract was evaluated with effective protection rate as index.Result：Supercritical CO2 extraction technology was adopted,optimum technology was as following：temperature 60 ℃,pressure 30 MPa,extraction time 2 h,yield of volatile oil approximately 0.35%.Effective protection rate of these prepared extract of A annua（concentration0.05 g·mL-1） reached 99% in 2 hours.Conclusion：This optimized technology was reasonable and stable and its extract had well repellent effect.