东北铁线莲中抗肿瘤活性成分的提取工艺优选

Optimization of Extraction Process of Anti-tumor Active Ingredient in Clematis manshurica Rupr

目的:建立东北铁线莲中抗肿瘤活性成分W2[3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃核糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖齐墩果酸皂苷]的提取工艺。方法:以W2提取量为指标,采用HPLC测定W2含量,通过正交试验和单因素试验考察提取溶媒、提取时间、料液比、提取次数对提取工艺的影响,确定W2最佳提取工艺。结果:最佳提取工艺为30%乙醇提取2.5 h,料液比1∶12,提取2次。结论:优选的提取工艺合理、稳定、可行,为东北铁线莲后续研究提供实验依据。

Objective：To establish extraction technology for anti-tumor active ingredient of W2 [3-O-α-L-pyran rhamnose-（1→6）-β-D-glucopyranosyl-（1→4）-β-D-glucopyranosyl-（1→4）-β-D-ribopyranose-（1→3）-α-L-pyran rhamnose-（1→2）-α-L-arabinopyranosyl oleanolic acid saponins] from Clematis manshurica Rupr.Method：With the content of W2 as index,which was determined by HPLC,effects of extraction time,extraction solvent,liquid-solid ratio and extraction times on extraction technology were investigated by single factor test and orthogonal test,then to determine optimum technology of W2.Result：Optimum extraction process of W2 was as following：reflux extracted 2 times with 12 times the amount of 30% ethanol,2.5 hours per time.Conclusion：Optimized technology was reasonable,stable and feasible,which could provide a experimental basis for the follow-up research of C.manshurica Rupr.