桂郁金水提物对大鼠肝胞浆和微粒体内Ⅱ相脱毒酶的影响

Effects of Water Extract of Curcuma kwangsiensis on Detoxication Enzymes in Cytosol and Microsomes of Rat Liver

目的:研究桂郁金水提物对大鼠肝胞浆液和微粒体内Ⅱ脱毒酶的影响。方法:雄性SD大鼠,桂郁金水提液按(生药剂量)0.81,2.43,7.29 g·kg-13个剂量组,灌胃给药,1次/d,连续3周,末次给药后,动物取血,分离血清,测丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、尿素氮(BUN)、肌酐(Cr),摘除肝脏,差速离心法制备肝脏胞浆液及微粒体,测定胞浆液的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)和微粒体内谷胱甘肽-S-转移酶(GST)和尿苷二磷酸-葡萄糖醛酸转移酶(UGT)的活性。结果:桂郁金水提液对大鼠体重增长和肝脏指数及血清ALT,AST含量均无明显影响;桂郁金各剂量组明显降低Cr含量(P<0.01);中、高剂量组明显降低了BUN含量(P<0.01)。在肝胞浆液,与对照组比较,桂郁金各剂量组对CAT和GR的活性没有明显影响;桂郁金各剂量均明显增高GSH-Px活性(P<0.01);桂郁金中、高剂量明显增高SOD活性(P<0.01,P<0.05)。在肝微粒体中,与对照组比较,桂郁金各剂量组对UGT的活性没有显著的影响;桂郁金各剂量均明显增高GST活性(P<0.05)。结论:桂郁金对大鼠肝脏和肾脏没有毒性,对肾脏可能还具有保护作用。桂郁金可诱导胞浆液和微粒体内脱毒酶的功能,具有抗氧化作用,并能加速体内毒性代谢物的排除,增加肝脏的解毒能力。

Objective：To study the effects of water extracts from Curcuma kwangsiensis on liver detoxication enzymes in cytosol and microsomes in rats.Method：Rats were treated with 0.81,2.43,7.29 g ·kg-1 once daily for 21 days,serum were collected for determination of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,urea nitrogen（BUN）,creatinine（Cr）,and liver was removed,then cytosols and microsomes isolated from liver were prepared by differential centrifugation according to the standard procedure.Antioxidant enzmes catalase（CAT）,superoxide dismutase（SOD）,glutathione peroxidose（GSH-Px）,glutathione reductase（Gr） in cytosols and glutathione-S-transferase（GST）,uridinediphosphoglucuronate glucuronosyl transferase（UGT） in microsomes were determined by UV-V is spectrophotometer.Result：C.kwangsiensis water extract had no effect on weight,liversomatic indexes,ALT and AST activities under different treatment compared with control group（P0.05）,but significantly decreased the level of Cr（P0.01） in all groups,middle and high doses could also decreased BUN level（P0.01）.Compared with the control group,C.kwangsiensis had no effect on CAT and GR in cytosols.The groups of 2.43 g·kg-1（P0.01） and 7.29 g·kg-1（P0.05） significantly increased the activities of SOD.Furthermore,all of the C.kwangsiensis groups significantly increased the activities of GSH-Px（P0.01）.Compared with the control group,all of C.kwangsiensis groups had no effect on the activities of UGT.All groups of C.kwangsiensis,significantly induced the activities of GST（P0.05） in microsomes.Conclusion：C.kwangsiensis have no toxicity on liver and kidney in rats,on the contrary,C.kwangsiensis may have a protective effect on kidney.C.kwangsiensis can induce the detoxication enzyames in cytosols and microsomes of rat liver,it have antioxidant function and increase the detoxication of liver.