何首乌与制何首乌补血作用及HPLC指纹图谱的比较

Comparison on Effect of Anemia Animal Model and HPLC Fingerprint Chromatogram between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata

目的:通过比较何首乌、制何首乌水煎液的补血作用及其HPLC指纹图谱,初步探索两者药效不同的物质基础。方法:将大鼠分为4组,除正常组外,对其他3组大鼠于实验第2和第6天分别sc 0.015,0.03 g·kg-1剂量的乙酰苯肼,并于第6天起每天ip 0.03 g·kg-1剂量环磷酰胺,持续3 d,建立大鼠贫血动物模型,并于造模期间每天对相应组别大鼠灌胃何首乌、制何首乌水煎液各2 g·kg-1,造模结束后取血检测给药后大鼠外周血象红细胞计数(RBC),血红蛋白测定(Hgb),红细胞压积(HCT)的变化。通过高效液相色谱分析法,制定并建立何首乌与制何首乌的HPLC指纹图谱。结果:与正常对照组比较,贫血模型组大鼠RBC,Hgb,HCT均显著降低(P<0.01),说明造模成功;何首乌组大鼠RBC,Hgb,HCT与贫血模型组比较,差异无统计学意义,仅HCT显著高于模型组(P<0.05);制何首乌组大鼠RBC,Hgb,HCT与模型组比较,均显著升高(P<0.01或P<0.05)。指纹图谱比对则发现制何首乌与何首乌有成分的差异,同时也有共有成分含量的差异。结论:制何首乌具有显著的补血功效,研究结果提示制何首乌中所含有的新的化学成分很有可能为麦拉德反应的产物,制何首乌较何首乌具有更佳补血功效有可能是该部分产物与较为温和的药性共同作用的结果。

Objective：Tocomparie effect of blood deficiency and HPLC fingerprint chromatogram between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata,and to findout the material foundationof effects.Method：According to random design,rats were divided into 4 groups.Except the normal control group,the other groups were subcutaneously injected acetyl phenyl hydrazine 0.015 g·kg-1 and 0.03 g·kg-1 in the 3rd day and the 6th day.From the 6th day,rats were intraperitoneally injected CTX for 3 day.During the period of modeling,treatment groups received corresponding drugs at dose of 2 g·kg-1.Then,the effect of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata were observed,and the fingerprint chromatogram of the water extracted from Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata,respectively,were established by HPLC.Result：Compared with the normal control group,the results showed that the red blood cell count（RBC）,hemoglobin（Hgb）,hernatocrit（HCT） figures in model group rats declined significantly（P0.05）.Compared with the model group,the results showed that Polygoni Multiflori Radix could not increase RBC and Hgb in anemia model rats,but the HCT was increased（P0.05）,while Polygoni Multiflori Radix Preparata could rise RBC Hgb and HCT significantly in anemia model rats（P0.01 or P0.05）.The fingerprint chromatograms result revealed that there were differences not only in chemical consistence but in n determination of constituents between two herbals.Conclusion：The results illustrate that Polygoni Multiflori Radix Preparata has significant blood-nourishing ability.Additionally,the result of the fingerprint chromatogram indicates that the product of Maillard reaction and moderate drug properties are both the possible reason why Polygoni Multiflori Radix Preparata has the better efficacy on blood deficiency.