小柴胡汤对功能性消化不良大鼠血管活性肠肽、胃排空及小肠推进率的影响

Effects of Xiaochaihu Decoction on VIP,Gastric Emptying and Small Intestine Advance Rate in Functional Dyspepsia Rats

目的:观察小柴胡汤对功能性消化不良(FD)大鼠血管活性肠肽(VIP)、胃排空及小肠推进率的影响。方法:将48只SD大鼠随机分为空白组、模型组、小柴胡汤组和多潘立酮组,每组12只。采用慢性束缚应激+过度疲劳+饮食失节的方法造模,每天1次,连续3周。造模结束后,分别对4组大鼠进行相应被试因素的灌胃处理14 d。灌胃结束后,分别测定各组VIP、胃内残留率及小肠推进率。结果:与空白组相比,模型组大鼠血清VIP含量及胃内残留率明显升高,小肠推进率显著降低,且均有显著性差异(P<0.05或P<0.01);与模型组相比,小柴胡汤组和多潘立酮组大鼠血清VIP含量及胃内残留率显著降低,小肠推进率明显升高,且均有显著性差异(P<0.05或P<0.01)。结论:小柴胡汤可降低FD模型大鼠血清中VIP的含量,加速胃排空及小肠推进率。

Objective：To observe the effects of Xiaochaihu decoction on vasoactive intestinal peptide（VIP）,gastric emptying and small intestine advance rate in functional dyspepsia（FD） rats.Method：48 rats were randomly divided into four groups：blank group,model group,Xiaochaihu decoction group and domperidone group,each group had 12 rats.FD models were established by chronic restraint stress and excessive fatigue and irregular feeding,once per day for continuous 3 weeks.Then feed the rats in different groups with normal saline,normal saline,Xiaochaihu decoction and Domperidone liquor respectively by intragastric administration for continuous 14 days.After 14 days intragastric administration,VIP of each rat was detected.Gastric emptying and small intestine advance rate of each rat were tested.Result：Compared to the blank group,gastrointestinal Hormone VIP and gastric residue obviously increased while small intestine advance rate significantly decreased in model group（P0.05 or P0.01）.Compared to the model group,gastrointestinal hormone VIP and gastric residue obviously decreased while small intestine advance rate significantly increased in the Xiaochaihu decoction and domperidone group（P0.05 or P0.01）.Conclusion：Xiaochaihu decoction can reduce gastrointestinal hormone VIP,advance gastric emptying and small intestine advance rate in functional dyspepsia rats.